{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kolster M"],"funding":["Xunta de Galicia","Horizon 2020 Framework Programme","European Regional Development Fund"],"pagination":["36095-36105"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11261559"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(28)"],"pubmed_abstract":["Gene therapies represent promising new therapeutic options for a variety of indications. However, despite several approved drugs, its potential remains untapped. For polymeric gene delivery, endosomal escape represents a bottleneck. SO1861, a naturally occurring triterpene saponin with endosomal escape properties isolated from <i>Saponaria officinalis</i> L., has been described as additive agent to enhance transfection efficiency (sapofection). However, the challenge to synchronize the saponin and gene delivery system <i>in vivo</i> imposes limitations. Herein, we address this issue by conjugating SO1861 to a peptide-based gene vector using a pH-sensitive hydrazone linker programmed to release SO1861 at the acidic pH of the endosome. Nanoplexes formulated with SO1861-equipped peptides were"],"journal":["ACS applied materials & interfaces"],"pubmed_title":["Broadening the Scope of Sapofection: Cationic Peptide-Saponin Conjugates Improve Gene Delivery <i>In Vitro</i> and <i>In Vivo</i>."],"pmcid":["PMC11261559"],"funding_grant_id":["825730","ED431G 2023/03","ED431C 2022/21"],"pubmed_authors":["Weng A","Correa J","Fernandez-Megia E","Weise C","Fuchs H","Sonntag A","Walther W","Kolster M"],"additional_accession":[]},"is_claimable":false,"name":"Broadening the Scope of Sapofection: Cationic Peptide-Saponin Conjugates Improve Gene Delivery <i>In Vitro</i> and <i>In Vivo</i>.","description":"Gene therapies represent promising new therapeutic options for a variety of indications. However, despite several approved drugs, its potential remains untapped. For polymeric gene delivery, endosomal escape represents a bottleneck. SO1861, a naturally occurring triterpene saponin with endosomal escape properties isolated from <i>Saponaria officinalis</i> L., has been described as additive agent to enhance transfection efficiency (sapofection). However, the challenge to synchronize the saponin and gene delivery system <i>in vivo</i> imposes limitations. Herein, we address this issue by conjugating SO1861 to a peptide-based gene vector using a pH-sensitive hydrazone linker programmed to release SO1861 at the acidic pH of the endosome. Nanoplexes formulated with SO1861-equipped peptides were","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2026-06-01T20:38:28.825Z","creation":"2025-06-01T01:16:03.907Z"},"accession":"S-EPMC11261559","cross_references":{"pubmed":["38970470"],"doi":["10.1021/acsami.4c05846"]}}