biostudies-literatureUnknown15Kim HD<h4>Introduction</h4>The aim of this study is to investigate the clinical validity of donor-derived cell-free DNA (dd-cfDNA) in comparison with that of donor specific anti-HLA antibody (DSA) for predicting biopsy-proven rejection (BPR)and severe microvascular inflammation (severe MVI) in kidney transplant recipients (KTRs).<h4>Methods</h4>In this prospective observational investigation, 64 KTRs who underwent the indicated biopsies were included. Blood samples collected prior to biopsy were tested for dd-cfDNA and DSA. Biopsy specimens were classified by a renal pathologist according to the Banff classification. The predictive performance of dd-cfDNA and DSA for histological allograft diagnosis was assessed.<h4>Results</h4>KTRs were categorized into the high and low dd-cfDNA groups based onFrontiers in immunology1433918https://www.ebi.ac.uk/biostudies/studies/S-EPMC11263016biostudies-literatureDonor-derived cell-free DNA predicted allograft rejection and severe microvascular inflammation in kidney transplant recipients.PMC11263016Bae HKang HOh EJChung BHChoi YJKim HDEum SHLee HYang CWfalseDonor-derived cell-free DNA predicted allograft rejection and severe microvascular inflammation in kidney transplant recipients.<h4>Introduction</h4>The aim of this study is to investigate the clinical validity of donor-derived cell-free DNA (dd-cfDNA) in comparison with that of donor specific anti-HLA antibody (DSA) for predicting biopsy-proven rejection (BPR)and severe microvascular inflammation (severe MVI) in kidney transplant recipients (KTRs).<h4>Methods</h4>In this prospective observational investigation, 64 KTRs who underwent the indicated biopsies were included. Blood samples collected prior to biopsy were tested for dd-cfDNA and DSA. Biopsy specimens were classified by a renal pathologist according to the Banff classification. The predictive performance of dd-cfDNA and DSA for histological allograft diagnosis was assessed.<h4>Results</h4>KTRs were categorized into the high and low dd-cfDNA groups based on2024-01-01T00:00:00Z20242025-06-01T01:16:48.579Z2025-06-01T01:16:48.579ZS-EPMC112630163904481710.3389/fimmu.2024.1433918