biostudies-literatureXu YHealth Scientific Research Innovation Ability Improvement Plan in Shaanxi Province of ChinaNatural Science Basic Research Plan in Shaanxi Province of ChinaKey R&D plan of Shaanxi ProvinceKey R&D plan of Shaanxi ProvinceThe National Natural Science Foundation of ChinaThe National Natural Science Foundation of China (NSFC)1232-1245https://www.ebi.ac.uk/biostudies/studies/S-EPMC11263418biostudies-literatureUnknown29(7-8)MARCH5 is a ring-finger E3 ubiquitin ligase located in the outer membrane of mitochondria. A previous study has reported that MARCH5 was up-regulated and contributed to the migration and invasion of OC cells by serving as a competing endogenous RNA. However, as a mitochondrial localized E3 ubiquitin ligase, the function of MARCH5 in mitochondrial-associated metabolism reprogramming in human cancers remains largely unexplored, including OC. We first assessed the glycolysis effect of MARCH5 in OC both in vitro and in vivo. Then we analyzed the effect of MARCH5 knockdown or overexpression on respiratory activity by evaluating oxygen consumption rate, activities of OXPHOS complexes and production of ATP in OC cells with MARCH5. Co-immunoprecipitation, western-blot, and in vitro and vivo experiApoptosis : an international journal on programmed cell deathMARCH5 promotes aerobic glycolysis to facilitate ovarian cancer progression via ubiquitinating MPC1.PMC112634182022JQ-9772023PT-07S2023-YF-YBSF-0816NO.821729932024JC-YBMS-703Yang HLi YLi JZhao SChen ZGuo FShen YDang YXu YfalseMARCH5 promotes aerobic glycolysis to facilitate ovarian cancer progression via ubiquitinating MPC1.MARCH5 is a ring-finger E3 ubiquitin ligase located in the outer membrane of mitochondria. A previous study has reported that MARCH5 was up-regulated and contributed to the migration and invasion of OC cells by serving as a competing endogenous RNA. However, as a mitochondrial localized E3 ubiquitin ligase, the function of MARCH5 in mitochondrial-associated metabolism reprogramming in human cancers remains largely unexplored, including OC. We first assessed the glycolysis effect of MARCH5 in OC both in vitro and in vivo. Then we analyzed the effect of MARCH5 knockdown or overexpression on respiratory activity by evaluating oxygen consumption rate, activities of OXPHOS complexes and production of ATP in OC cells with MARCH5. Co-immunoprecipitation, western-blot, and in vitro and vivo experi2024-01-01T00:00:00Z2024 Aug2025-06-01T01:15:39.98Z2025-06-01T01:15:39.98ZS-EPMC112634183861508310.1007/s10495-024-01962-5