{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kessler LF"],"funding":["Deutsche Forschungsgemeinschaft","Goethe-Universit?t Frankfurt am Main"],"pagination":["6751-6759"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11264260"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["128(28)"],"pubmed_abstract":["Protein labeling through transient and repetitive hybridization of short, fluorophore-labeled DNA oligonucleotides has become widely applied in various optical super-resolution microscopy methods. The main advantages are multitarget imaging and molecular quantification. A challenge is the high background signal originating from the presence of unbound fluorophore-DNA labels in solution. Here, we report the self-quenching of fluorophore dimers conjugated to DNA oligonucleotides as a general concept to reduce the fluorescence background. Upon hybridization, the fluorescence signals of both fluorophores are restored. We expand the toolbox of fluorophores suitable for self-quenching and report their spectra and hybridization equilibria. We apply self-quenched fluorophore-DNA labels to stimulat"],"journal":["The journal of physical chemistry. B"],"pubmed_title":["Self-Quenched Fluorophore-DNA Labels for Super-Resolution Fluorescence Microscopy."],"pmcid":["PMC11264260"],"funding_grant_id":["CRC 1177","INST 161/1020-1 FUGG","CRC 1507"],"pubmed_authors":["Mantel M","Li Y","Dietz MS","Menche T","Wang D","Heilemann M","Kessler LF","Balakrishnan A","Glogger M"],"additional_accession":[]},"is_claimable":false,"name":"Self-Quenched Fluorophore-DNA Labels for Super-Resolution Fluorescence Microscopy.","description":"Protein labeling through transient and repetitive hybridization of short, fluorophore-labeled DNA oligonucleotides has become widely applied in various optical super-resolution microscopy methods. The main advantages are multitarget imaging and molecular quantification. A challenge is the high background signal originating from the presence of unbound fluorophore-DNA labels in solution. Here, we report the self-quenching of fluorophore dimers conjugated to DNA oligonucleotides as a general concept to reduce the fluorescence background. Upon hybridization, the fluorescence signals of both fluorophores are restored. We expand the toolbox of fluorophores suitable for self-quenching and report their spectra and hybridization equilibria. We apply self-quenched fluorophore-DNA labels to stimulat","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2025-06-01T01:15:48.184Z","creation":"2025-06-01T01:15:48.184Z"},"accession":"S-EPMC11264260","cross_references":{"pubmed":["38955346"],"doi":["10.1021/acs.jpcb.4c02065"]}}