{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li N"],"funding":["American Heart Association","American Heart Association-American Stroke Association","South Dakota Board of Regents"],"pagination":["61-69"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11264340"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["93"],"pubmed_abstract":["Construction of an artificial vascular graft is widely considered a promising strategy in vascular tissue engineering. However, limited sources of functional vascular smooth muscle cells (VSMCs) remain a major obstacle in vascular tissue engineering. In this study, we innovatively developed an approach to obtain functional VSMCs by onsite differentiating human bone marrow-derived mesenchymal stem cells (MSCs) directed by decellularized extracellular matrix (ECM) and fibroblasts. The resulting cells and ECM-cells constructs were characterized by real time RT-PCR, immunofluorescence staining, cell contractile functions, and migration capacity. Our results showed both ECM and fibroblasts induced MSCs differentiation toward VSMC-like cells with increased transcription of marker genes, upregula"],"journal":["Materials science & engineering. C, Materials for biological applications"],"pubmed_title":["Vascular extracellular matrix and fibroblasts-coculture directed differentiation of human mesenchymal stem cells toward smooth muscle-like cells for vascular tissue engineering."],"pmcid":["PMC11264340"],"funding_grant_id":["15SDG25420001","UP1600205"],"pubmed_authors":["Hong Z","Sanyour H","Kelly P","Li N","Remund T"],"additional_accession":[]},"is_claimable":false,"name":"Vascular extracellular matrix and fibroblasts-coculture directed differentiation of human mesenchymal stem cells toward smooth muscle-like cells for vascular tissue engineering.","description":"Construction of an artificial vascular graft is widely considered a promising strategy in vascular tissue engineering. However, limited sources of functional vascular smooth muscle cells (VSMCs) remain a major obstacle in vascular tissue engineering. In this study, we innovatively developed an approach to obtain functional VSMCs by onsite differentiating human bone marrow-derived mesenchymal stem cells (MSCs) directed by decellularized extracellular matrix (ECM) and fibroblasts. The resulting cells and ECM-cells constructs were characterized by real time RT-PCR, immunofluorescence staining, cell contractile functions, and migration capacity. Our results showed both ECM and fibroblasts induced MSCs differentiation toward VSMC-like cells with increased transcription of marker genes, upregula","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Dec","modification":"2025-05-18T13:26:43.826Z","creation":"2025-05-18T13:26:43.826Z"},"accession":"S-EPMC11264340","cross_references":{"pubmed":["30274093"],"doi":["10.1016/j.msec.2018.07.061"]}}