{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["17(1)"],"submitter":["Zhang H"],"pubmed_abstract":["<h4>Background</h4>Polycystic ovarian syndrome (PCOS) accounts for about 75% of anovulatory infertility. The cause of PCOS is not clear. CircRNAs acting as miRNA sponges mediate the post-transcriptional regulation of multiple genes. CYP19A1 is a limiting enzyme in the ovarian steroidogenesis pathway. However, the mechanism of circRNAs regulating granulosa cell (GC) estradiol secretion in PCOS remains to be elucidated.<h4>Methods</h4>Bioinformatics was used to predict the potential target miRNAs of circ_0043532 and target genes of miR-1270. Target miRNAs and mRNA expression were verified by qRT-PCR in GCs from 45 women with PCOS and 65 non-PCOS. Western blot, ELISA and dual-luciferase reporter assays were applied to confirm the substrate of miR-1270.<h4>Results</h4>Circ_0043532 and CYP19A1 "],"journal":["Journal of ovarian research"],"pagination":["151"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11265019"],"repository":["biostudies-literature"],"pubmed_title":["Hsa_circ_0043532 contributes to PCOS through upregulation of CYP19A1 by acting as a ceRNA for hsa-miR-1270."],"pmcid":["PMC11265019"],"pubmed_authors":["Liu Y","Fang J","Li D","Zhang Y","Zhang H","Zhu W","Hao C","Shen W","Xu Y"],"additional_accession":[]},"is_claimable":false,"name":"Hsa_circ_0043532 contributes to PCOS through upregulation of CYP19A1 by acting as a ceRNA for hsa-miR-1270.","description":"<h4>Background</h4>Polycystic ovarian syndrome (PCOS) accounts for about 75% of anovulatory infertility. The cause of PCOS is not clear. CircRNAs acting as miRNA sponges mediate the post-transcriptional regulation of multiple genes. CYP19A1 is a limiting enzyme in the ovarian steroidogenesis pathway. However, the mechanism of circRNAs regulating granulosa cell (GC) estradiol secretion in PCOS remains to be elucidated.<h4>Methods</h4>Bioinformatics was used to predict the potential target miRNAs of circ_0043532 and target genes of miR-1270. Target miRNAs and mRNA expression were verified by qRT-PCR in GCs from 45 women with PCOS and 65 non-PCOS. Western blot, ELISA and dual-luciferase reporter assays were applied to confirm the substrate of miR-1270.<h4>Results</h4>Circ_0043532 and CYP19A1 ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2025-06-01T01:15:54.444Z","creation":"2025-06-01T01:15:54.444Z"},"accession":"S-EPMC11265019","cross_references":{"pubmed":["39039600"],"doi":["10.1186/s13048-024-01474-5"]}}