biostudies-literatureJaroslawska JHorizon 2020 Framework ProgrammeNarodowe Centrum Naukie0306426https://www.ebi.ac.uk/biostudies/studies/S-EPMC11265685biostudies-literatureUnknown19(7)The vitamin D3 metabolite 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), its nuclear receptor VDR (vitamin D receptor) and hundreds of their target genes are not only key regulators of calcium homeostasis, but also important modulators of the immune system. Innate immune cells like monocytes use VDR for efficient differentiation and are very responsive to vitamin D. So far, most information on the gene regulatory function of vitamin D and its physiological impact had been obtained from in vitro studies using supraphysiological doses of 1,25(OH)2D3. Therefore, medical experiments like the study VitDHiD (NCT03537027), where 25 healthy individuals were supplemented once with a vitamin D3 bolus (80,000 IU), provide important insight into the response to vitamin D under in vivo conditions. In this stuPloS oneIn vivo vitamin D target genes interconnect key signaling pathways of innate immunity.PMC112656859526012023/49/B/NZ9/00402Ghosh Dastidar RCarlberg CJaroslawska JfalseIn vivo vitamin D target genes interconnect key signaling pathways of innate immunity.The vitamin D3 metabolite 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), its nuclear receptor VDR (vitamin D receptor) and hundreds of their target genes are not only key regulators of calcium homeostasis, but also important modulators of the immune system. Innate immune cells like monocytes use VDR for efficient differentiation and are very responsive to vitamin D. So far, most information on the gene regulatory function of vitamin D and its physiological impact had been obtained from in vitro studies using supraphysiological doses of 1,25(OH)2D3. Therefore, medical experiments like the study VitDHiD (NCT03537027), where 25 healthy individuals were supplemented once with a vitamin D3 bolus (80,000 IU), provide important insight into the response to vitamin D under in vivo conditions. In this stu2024-01-01T00:00:00Z20242026-07-15T09:34:38.111Z2025-04-19T13:14:08.18ZS-EPMC112656853904261310.1371/journal.pone.0306426