biostudies-literatureCheng XNHLBI NIH HHSNational Institutes of HealthNIGMS NIH HHS101977https://www.ebi.ac.uk/biostudies/studies/S-EPMC11267048biostudies-literatureUnknown86<h4>Objective</h4>The prevalence of metabolic dysfunction-associated steatotic liver disease (MASLD) continues to rise with the increasing obesity epidemic. Rezdiffra as an activator of a thyroid hormone receptor-beta is the only Food and Drug Administration approved therapy. As such, there is a critical need to improve our understanding of gene expression regulation and signaling transduction in MASLD to develop new therapies. Matrin-3 is a DNA- and RNA-binding protein involved in the pathogenesis of human diseases. Here we examined its previously uncharacterized role in limiting hepatic steatosis and stress response via the constitutive androstane receptor (CAR).<h4>Methods</h4>Matrin-3 floxed and liver-specific knockout mice were fed either a chow diet or 60 kcal% high-fat diet (HFD) foMolecular metabolismLiver matrin-3 protects mice against hepatic steatosis and stress response via constitutive androstane receptor.PMC11267048R01 GM147913R01 HL150536Zhao MRiethoven JJMoran MBaki VBLiu BLi QHarris ENGurumurth CBYu JSun XCheng XfalseLiver matrin-3 protects mice against hepatic steatosis and stress response via constitutive androstane receptor.<h4>Objective</h4>The prevalence of metabolic dysfunction-associated steatotic liver disease (MASLD) continues to rise with the increasing obesity epidemic. Rezdiffra as an activator of a thyroid hormone receptor-beta is the only Food and Drug Administration approved therapy. As such, there is a critical need to improve our understanding of gene expression regulation and signaling transduction in MASLD to develop new therapies. Matrin-3 is a DNA- and RNA-binding protein involved in the pathogenesis of human diseases. Here we examined its previously uncharacterized role in limiting hepatic steatosis and stress response via the constitutive androstane receptor (CAR).<h4>Methods</h4>Matrin-3 floxed and liver-specific knockout mice were fed either a chow diet or 60 kcal% high-fat diet (HFD) fo2024-01-01T00:00:00Z2024 Aug2026-06-01T13:27:19.899Z2025-04-19T13:10:30.969ZS-EPMC112670483893665910.1016/j.molmet.2024.101977