biostudies-literatureUnknown90(7)Deschner DMinistry of Agriculture - Saskatchewan (Sask Agriculture)Ministry of Agriculture - SaskatchewanSaskatchewan Cattlemen's Association (SCA)Saskatchewan Cattlemen&apos;s Association<i>Mannheimia haemolytica</i> is a major contributor to bovine respiratory disease (BRD), which causes substantial economic losses to the beef industry, and there is an urgent need for rapid and accurate diagnostic tests to provide evidence for treatment decisions and support antimicrobial stewardship. Diagnostic sequencing can provide information about antimicrobial resistance genes in <i>M. haemolytica</i> more rapidly than conventional diagnostics. Realizing the full potential of diagnostic sequencing requires a comprehensive understanding of the genetic markers of antimicrobial resistance. We identified genetic markers of resistance in <i>M. haemolytica</i> to macrolide class antibiotics commonly used for control of BRD. Genome sequences were determined for 99 <i>M. haemolytica</i> isoApplied and environmental microbiologye0050224https://www.ebi.ac.uk/biostudies/studies/S-EPMC11267883biostudies-literatureIdentification of genetic markers of resistance to macrolide class antibiotics in <i>Mannheimia haemolytica</i> isolates from a Saskatchewan feedlot.PMC11267883Campbell JVoordouw MJWaldner CLHill JEFernando CDeschner DfalseIdentification of genetic markers of resistance to macrolide class antibiotics in <i>Mannheimia haemolytica</i> isolates from a Saskatchewan feedlot.<i>Mannheimia haemolytica</i> is a major contributor to bovine respiratory disease (BRD), which causes substantial economic losses to the beef industry, and there is an urgent need for rapid and accurate diagnostic tests to provide evidence for treatment decisions and support antimicrobial stewardship. Diagnostic sequencing can provide information about antimicrobial resistance genes in <i>M. haemolytica</i> more rapidly than conventional diagnostics. Realizing the full potential of diagnostic sequencing requires a comprehensive understanding of the genetic markers of antimicrobial resistance. We identified genetic markers of resistance in <i>M. haemolytica</i> to macrolide class antibiotics commonly used for control of BRD. Genome sequences were determined for 99 <i>M. haemolytica</i> iso2024-01-01T00:00:00Z2024 Jul2026-06-01T17:36:57.84Z2025-04-06T00:36:15.074ZS-EPMC112678833886463010.1128/aem.00502-24