biostudies-literatureMa YMOST | National Natural Science Foundation of China (NSFC)Tianjin Municipal Education CommissionKey laboratory of Marine Resource Chemistry and Food Technology, Ministry of EducationMOST | National Natural Science Foundation of Chinae0054024https://www.ebi.ac.uk/biostudies/studies/S-EPMC11267897biostudies-literatureUnknown90(7)Halophilic archaea are promising microbial cell factories for bacterioruberin (BR) production. BR is a natural product with multi-bioactivities, allowing potential application in many fields. In the previous work, a haloarchaeon <i>Halorubrum</i> sp. HRM-150 with a high proportion of BR (about 85%) was isolated, but the low yield impeded its large-scale production. This work figured out BR synthesis characteristics and mechanisms, and proposed strategies for yield improvement. First, glucose (10 g/L) and tryptone (15 g/L) were tested to be better sources for BR production. Besides, the combination of glucose and starch achieved the diauxic growth, and the biomass and BR productivity increased by 85% and 54% than using glucose. Additionally, this work first proposed the BR synthesis patternApplied and environmental microbiologyThe biosynthesis mechanism of bacterioruberin in halophilic archaea revealed by genome and transcriptome analysis.PMC11267897423061062019KJ220EMTUST-21-01Yang HXie WSong MMa YSui LZhang BSun ZfalseThe biosynthesis mechanism of bacterioruberin in halophilic archaea revealed by genome and transcriptome analysis.Halophilic archaea are promising microbial cell factories for bacterioruberin (BR) production. BR is a natural product with multi-bioactivities, allowing potential application in many fields. In the previous work, a haloarchaeon <i>Halorubrum</i> sp. HRM-150 with a high proportion of BR (about 85%) was isolated, but the low yield impeded its large-scale production. This work figured out BR synthesis characteristics and mechanisms, and proposed strategies for yield improvement. First, glucose (10 g/L) and tryptone (15 g/L) were tested to be better sources for BR production. Besides, the combination of glucose and starch achieved the diauxic growth, and the biomass and BR productivity increased by 85% and 54% than using glucose. Additionally, this work first proposed the BR synthesis pattern2024-01-01T00:00:00Z2024 Jul2026-06-01T19:46:36.149Z2025-04-04T01:21:10.201ZS-EPMC112678973882905410.1128/aem.00540-24