{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wei Y"],"funding":["Department of Science and Technology of Shandong Province","National Science Foundation of Shandong Province","MOST | National Natural Science Foundation of China (NSFC)","Shandong University","MOST | National Natural Science Foundation of China","Taishan Scholars Project of Shandong Province","Shandong University (SDU)"],"pagination":["e0089024"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11267941"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["90(7)"],"pubmed_abstract":["Biological valorization of lignin, the second most abundant biopolymer on Earth, is an indispensable sector to build a circular economy and net-zero future. However, lignin is recalcitrant to bioupcycling, demanding innovative solutions. We report here the biological valorization of lignin-derived aromatic carbon to value-added chemicals without requesting extra organic carbon and freshwater via reprogramming the marine <i>Roseobacter</i> clade bacterium <i>Roseovarius nubinhibens</i>. We discovered the unusual advantages of this strain for the oxidation of lignin monomers and implemented a CRISPR interference (CRISPRi) system with the <i>lacI</i>-P<sub>trc</sub> inducible module, nuclease-deactivated Cas9, and programmable gRNAs. This is the first CRISPR-based regulatory system in <i>R. n"],"journal":["Applied and environmental microbiology"],"pubmed_title":["Blue valorization of lignin-derived monomers via reprogramming marine bacterium <i>Roseovarius nubinhibens</i>."],"pmcid":["PMC11267941"],"funding_grant_id":["22278246","tstp20230604","ZR2021ME066","2022HWYQ-017","U20A20146, 22378233","Qilu Young Scholar Program"],"pubmed_authors":["Wang S-G","Wei Y","Xia P-F"],"additional_accession":[]},"is_claimable":false,"name":"Blue valorization of lignin-derived monomers via reprogramming marine bacterium <i>Roseovarius nubinhibens</i>.","description":"Biological valorization of lignin, the second most abundant biopolymer on Earth, is an indispensable sector to build a circular economy and net-zero future. However, lignin is recalcitrant to bioupcycling, demanding innovative solutions. We report here the biological valorization of lignin-derived aromatic carbon to value-added chemicals without requesting extra organic carbon and freshwater via reprogramming the marine <i>Roseobacter</i> clade bacterium <i>Roseovarius nubinhibens</i>. We discovered the unusual advantages of this strain for the oxidation of lignin monomers and implemented a CRISPR interference (CRISPRi) system with the <i>lacI</i>-P<sub>trc</sub> inducible module, nuclease-deactivated Cas9, and programmable gRNAs. This is the first CRISPR-based regulatory system in <i>R. n","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2025-04-04T02:14:05.063Z","creation":"2025-04-04T02:14:05.063Z"},"accession":"S-EPMC11267941","cross_references":{"pubmed":["38940564"],"doi":["10.1128/aem.00890-24"]}}