biostudies-literatureCarrico AWNIMHNIDA NIH HHSNIAID NIH HHSNIMH NIH HHSNIDANIAID151-158https://www.ebi.ac.uk/biostudies/studies/S-EPMC11269022biostudies-literatureUnknown120<h4>Objective</h4>This epigenomics sub-study embedded within a randomized controlled trial examined whether an evidenced-based behavioral intervention model that decreased stimulant use altered leukocyte DNA methylation (DNAm).<h4>Methods</h4>Sexual minority men with HIV who use methamphetamine were randomized to a five-session positive affect intervention (n = 32) or an attention-control condition (n = 21), both delivered during three months of contingency management for stimulant abstinence. All participants exhibited sustained HIV virologic control - an HIV viral load less than 40 copies/mL at baseline and six months post-randomization. The Illumina EPIC BeadChip measured leukocyte methylation of cytosine-phosphate-guanosine (CpG) sites mapping onto five a priori candidate genes of inteBrain, behavior, and immunityA positive affect intervention alters leukocyte DNA methylation in sexual minority men with HIV who use methamphetamine.PMC11269022K24-MH093225K24 DA057874P30 AI073961K23-DA039800P30 AI027763K24 MH093225R01 DA058437R01 DA033854R01-DA033854K23 DA039800Moskowitz JTAsam KGhanooni DAouizerat BEGouse HCherenack EMDilworth SEPaul RHFlentje ARubin LHNeilands TBCarrico AWFuchs DChavez JVfalseA positive affect intervention alters leukocyte DNA methylation in sexual minority men with HIV who use methamphetamine.<h4>Objective</h4>This epigenomics sub-study embedded within a randomized controlled trial examined whether an evidenced-based behavioral intervention model that decreased stimulant use altered leukocyte DNA methylation (DNAm).<h4>Methods</h4>Sexual minority men with HIV who use methamphetamine were randomized to a five-session positive affect intervention (n = 32) or an attention-control condition (n = 21), both delivered during three months of contingency management for stimulant abstinence. All participants exhibited sustained HIV virologic control - an HIV viral load less than 40 copies/mL at baseline and six months post-randomization. The Illumina EPIC BeadChip measured leukocyte methylation of cytosine-phosphate-guanosine (CpG) sites mapping onto five a priori candidate genes of inte2024-01-01T00:00:00Z2024 Aug2026-03-18T13:30:01.005Z2025-08-18T09:54:17.984ZS-EPMC112690223877728310.1016/j.bbi.2024.05.025