biostudies-literatureHao JNIAID NIH HHSNCI NIH HHSNational Institutes of HealthNIH HHS119https://www.ebi.ac.uk/biostudies/studies/S-EPMC11270797biostudies-literatureUnknown26(1)<h4>Background</h4>Breast cancer is the most common cancer in women diagnosed in the U.S. and worldwide. Obesity increases breast cancer risk without clear underlying molecular mechanisms. Our studies demonstrate that circulating adipose fatty acid binding protein (A-FABP, or FABP4) links obesity-induced dysregulated lipid metabolism and breast cancer risk, thus potentially offering a new target for breast cancer treatment.<h4>Methods</h4>We immunized FABP4 knockout mice with recombinant human FABP4 and screened hybridoma clones with specific binding to FABP4. The potential effects of antibodies on breast cancer cells in vitro were evaluated using migration, invasion, and limiting dilution assays. Tumor progression in vivo was evaluated in various types of tumorigenesis models including C5Breast cancer research : BCRDevelopment of a humanized anti-FABP4 monoclonal antibody for potential treatment of breast cancer.PMC11270797R01AI137324P30 CA086862R01 AI137324R01 CA180986U01 CA272424Chimenti MSSugg SLSchnicker NJYi YHao JJin RJiang XLi BYu JXu ZfalseDevelopment of a humanized anti-FABP4 monoclonal antibody for potential treatment of breast cancer.<h4>Background</h4>Breast cancer is the most common cancer in women diagnosed in the U.S. and worldwide. Obesity increases breast cancer risk without clear underlying molecular mechanisms. Our studies demonstrate that circulating adipose fatty acid binding protein (A-FABP, or FABP4) links obesity-induced dysregulated lipid metabolism and breast cancer risk, thus potentially offering a new target for breast cancer treatment.<h4>Methods</h4>We immunized FABP4 knockout mice with recombinant human FABP4 and screened hybridoma clones with specific binding to FABP4. The potential effects of antibodies on breast cancer cells in vitro were evaluated using migration, invasion, and limiting dilution assays. Tumor progression in vivo was evaluated in various types of tumorigenesis models including C52024-01-01T00:00:00Z2024 Jul2026-06-01T09:02:45.45Z2025-08-31T03:04:51.366ZS-EPMC112707973905453610.1186/s13058-024-01873-y