{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kader Chowdhury QMM"],"funding":["HHS | NIH | National Institute of General Medical Sciences","NIAID NIH HHS","HHS | NIH | National Institute of Allergy and Infectious Diseases","HHS | NIH | National Institute of General Medical Sciences (NIGMS)","NIGMS NIH HHS","HHS | NIH | National Institute of Allergy and Infectious Diseases (NIAID)"],"pagination":["e0003324"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11270904"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["206(7)"],"pubmed_abstract":["<i>Listeria monocytogenes</i> is a foodborne bacterial pathogen that causes listeriosis. Positive regulatory factor A (PrfA) is a pleiotropic master activator of virulence genes of <i>L. monocytogenes</i> that becomes active upon the entry of the bacterium into the cytosol of infected cells. <i>L. monocytogenes</i> can survive and multiply at low temperatures; this is accomplished through the maintenance of appropriate membrane fluidity via branched-chain fatty acid (BCFA) synthesis. Branched-chain α-keto acid dehydrogenase (BKD), which is composed of four polypeptides encoded by <i>lpd</i>, <i>bkdA1</i>, <i>bkdA2</i>, and <i>bkdB</i>, is known to play a vital role in BCFA biosynthesis. Here, we constructed BKD-deficient <i>Listeria</i> strains by in-frame deletion of <i>lpd</i>, <i>bkdA1<"],"journal":["Journal of bacteriology"],"pubmed_title":["An insight into the role of branched-chain α-keto acid dehydrogenase (BKD) complex in branched-chain fatty acid biosynthesis and virulence of <i>Listeria monocytogenes</i>."],"pmcid":["PMC11270904"],"funding_grant_id":["P20 GM103646-09","P20 GM103646","1R15AI180880-01","R15 AI180880"],"pubmed_authors":["Islam S","Abdelhamed H","Ogunleye SC","Thu D","Wang S","Freitag NE","Narayanan L","Kader Chowdhury QMM","Lawrence ML"],"additional_accession":[]},"is_claimable":false,"name":"An insight into the role of branched-chain α-keto acid dehydrogenase (BKD) complex in branched-chain fatty acid biosynthesis and virulence of <i>Listeria monocytogenes</i>.","description":"<i>Listeria monocytogenes</i> is a foodborne bacterial pathogen that causes listeriosis. Positive regulatory factor A (PrfA) is a pleiotropic master activator of virulence genes of <i>L. monocytogenes</i> that becomes active upon the entry of the bacterium into the cytosol of infected cells. <i>L. monocytogenes</i> can survive and multiply at low temperatures; this is accomplished through the maintenance of appropriate membrane fluidity via branched-chain fatty acid (BCFA) synthesis. Branched-chain α-keto acid dehydrogenase (BKD), which is composed of four polypeptides encoded by <i>lpd</i>, <i>bkdA1</i>, <i>bkdA2</i>, and <i>bkdB</i>, is known to play a vital role in BCFA biosynthesis. Here, we constructed BKD-deficient <i>Listeria</i> strains by in-frame deletion of <i>lpd</i>, <i>bkdA1<","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2025-04-22T10:46:07.515Z","creation":"2025-04-05T23:41:46.094Z"},"accession":"S-EPMC11270904","cross_references":{"pubmed":["38899896"],"doi":["10.1128/jb.00033-24"]}}