biostudies-literatureKader Chowdhury QMMHHS | NIH | National Institute of General Medical SciencesNIAID NIH HHSHHS | NIH | National Institute of Allergy and Infectious DiseasesHHS | NIH | National Institute of General Medical Sciences (NIGMS)NIGMS NIH HHSHHS | NIH | National Institute of Allergy and Infectious Diseases (NIAID)e0003324https://www.ebi.ac.uk/biostudies/studies/S-EPMC11270904biostudies-literatureUnknown206(7)<i>Listeria monocytogenes</i> is a foodborne bacterial pathogen that causes listeriosis. Positive regulatory factor A (PrfA) is a pleiotropic master activator of virulence genes of <i>L. monocytogenes</i> that becomes active upon the entry of the bacterium into the cytosol of infected cells. <i>L. monocytogenes</i> can survive and multiply at low temperatures; this is accomplished through the maintenance of appropriate membrane fluidity via branched-chain fatty acid (BCFA) synthesis. Branched-chain α-keto acid dehydrogenase (BKD), which is composed of four polypeptides encoded by <i>lpd</i>, <i>bkdA1</i>, <i>bkdA2</i>, and <i>bkdB</i>, is known to play a vital role in BCFA biosynthesis. Here, we constructed BKD-deficient <i>Listeria</i> strains by in-frame deletion of <i>lpd</i>, <i>bkdA1<Journal of bacteriologyAn insight into the role of branched-chain α-keto acid dehydrogenase (BKD) complex in branched-chain fatty acid biosynthesis and virulence of <i>Listeria monocytogenes</i>.PMC11270904P20 GM103646-09P20 GM1036461R15AI180880-01R15 AI180880Islam SAbdelhamed HOgunleye SCThu DWang SFreitag NENarayanan LKader Chowdhury QMMLawrence MLfalseAn insight into the role of branched-chain α-keto acid dehydrogenase (BKD) complex in branched-chain fatty acid biosynthesis and virulence of <i>Listeria monocytogenes</i>.<i>Listeria monocytogenes</i> is a foodborne bacterial pathogen that causes listeriosis. Positive regulatory factor A (PrfA) is a pleiotropic master activator of virulence genes of <i>L. monocytogenes</i> that becomes active upon the entry of the bacterium into the cytosol of infected cells. <i>L. monocytogenes</i> can survive and multiply at low temperatures; this is accomplished through the maintenance of appropriate membrane fluidity via branched-chain fatty acid (BCFA) synthesis. Branched-chain α-keto acid dehydrogenase (BKD), which is composed of four polypeptides encoded by <i>lpd</i>, <i>bkdA1</i>, <i>bkdA2</i>, and <i>bkdB</i>, is known to play a vital role in BCFA biosynthesis. Here, we constructed BKD-deficient <i>Listeria</i> strains by in-frame deletion of <i>lpd</i>, <i>bkdA1<2024-01-01T00:00:00Z2024 Jul2025-04-22T10:46:07.515Z2025-04-05T23:41:46.094ZS-EPMC112709043889989610.1128/jb.00033-24