biostudies-literatureLiang NENIDDK NIH HHS540https://www.ebi.ac.uk/biostudies/studies/S-EPMC11273435biostudies-literatureUnknown13(7)Foreign body response (FBR) is a universal reaction to implanted biomaterial that can affect the function and longevity of the implant. A few studies have attempted to identify targets for treating FBR through the use of single-cell RNA sequencing (scRNA-seq), though the generalizability of these findings from an individual study may be limited. In our study, we perform a meta-analysis of scRNA-seq data from all available FBR mouse studies and integrate these data to identify gene signatures specific to FBR across different models and anatomic locations. We identify subclusters of fibroblasts and macrophages that emerge in response to foreign bodies and characterize their signaling pathways, gene ontology terms, and downstream mediators. The fibroblast subpopulations enriched in the settinBiologyUnderstanding the Foreign Body Response via Single-Cell Meta-Analysis.PMC11273435K08 DK134871Longaker MTLiang NEParker JBJanuszyk MWan DCGriffin MLu JMfalseUnderstanding the Foreign Body Response via Single-Cell Meta-Analysis.Foreign body response (FBR) is a universal reaction to implanted biomaterial that can affect the function and longevity of the implant. A few studies have attempted to identify targets for treating FBR through the use of single-cell RNA sequencing (scRNA-seq), though the generalizability of these findings from an individual study may be limited. In our study, we perform a meta-analysis of scRNA-seq data from all available FBR mouse studies and integrate these data to identify gene signatures specific to FBR across different models and anatomic locations. We identify subclusters of fibroblasts and macrophages that emerge in response to foreign bodies and characterize their signaling pathways, gene ontology terms, and downstream mediators. The fibroblast subpopulations enriched in the settin2024-01-01T00:00:00Z2024 Jul2026-04-08T19:21:21.053Z2025-04-19T13:10:57.877ZS-EPMC112734353905673310.3390/biology13070540