biostudies-literatureUnknown13(7)Musa LThe ability of <i>Salmonella</i> species to adhere to surfaces and form biofilms, leading to persistent environmental reservoirs, might represent a direct link between environmental contamination and food processing contamination. The purpose of this study was to investigate the biofilm-forming ability of 80 multidrug-resistant (MDR) and extended-spectrum beta-lactamase (ESBL) producing <i>Salmonella enterica</i> serovar Infantis strains isolated from the broiler food chain production through whole genome sequencing (WGS), PCR, and morphotype association assays. Biofilm formation was quantified by testing the strains at two different temperatures, using 96-well polystyrene plates. The rough and dry colony (rdar) morphotype was assessed visually on Congo red agar (CRA) plates. Based on our Antibiotics (Basel, Switzerland)595https://www.ebi.ac.uk/biostudies/studies/S-EPMC11273867biostudies-literatureHigh Biofilm-Forming Multidrug-Resistant <i>Salmonella</i> Infantis Strains from the Poultry Production Chain.PMC11273867Toppi VStefanetti VRapi MCAddis MFFranciosini MPCasagrande Proietti PMusa LGrilli GDi Giacinto GSpata NfalseHigh Biofilm-Forming Multidrug-Resistant <i>Salmonella</i> Infantis Strains from the Poultry Production Chain.The ability of <i>Salmonella</i> species to adhere to surfaces and form biofilms, leading to persistent environmental reservoirs, might represent a direct link between environmental contamination and food processing contamination. The purpose of this study was to investigate the biofilm-forming ability of 80 multidrug-resistant (MDR) and extended-spectrum beta-lactamase (ESBL) producing <i>Salmonella enterica</i> serovar Infantis strains isolated from the broiler food chain production through whole genome sequencing (WGS), PCR, and morphotype association assays. Biofilm formation was quantified by testing the strains at two different temperatures, using 96-well polystyrene plates. The rough and dry colony (rdar) morphotype was assessed visually on Congo red agar (CRA) plates. Based on our 2024-01-01T00:00:00Z2024 Jun2026-07-09T11:15:29.844Z2026-07-09T10:43:38.589ZS-EPMC112738673906127710.3390/antibiotics13070595