biostudies-literatureXian JUniversity of California System1573https://www.ebi.ac.uk/biostudies/studies/S-EPMC11274362biostudies-literatureUnknown12(7)Drs. John and Ford reported in biomedicines that a variant transcript encoding receptor tyrosine kinase-like orphan receptor 1 (ROR1), namely ENST00000545203 or variant 3 (ROR1V3), was a predominant ROR1 transcript of neoplastic or normal cells in the Bioinformatic database, including GTEx and the 33 datasets from TCGA. Unlike the full-length ROR1 transcript, Drs. John and Ford deduced that ROR1V3 encoded a cytoplasmic ROR1 protein lacking an apparent signal peptide necessary for transport to the cell surface, which they presumed made it unlikely to function as a surface receptor for Wingless/Integrated (Wnt) factors. Moreover, they speculated that studies evaluating ROR1 via immunohistochemistry using any one of several anti-ROR1 mABiomedicinesVariant Transcript of ROR1 ENST00000545203 Does Not Encode ROR1 Protein.PMC112743621007844Girgis CKipps TJXian JWidhopf GFCring MROh CSSinha NfalseVariant Transcript of ROR1 ENST00000545203 Does Not Encode ROR1 Protein.Drs. John and Ford reported in biomedicines that a variant transcript encoding receptor tyrosine kinase-like orphan receptor 1 (ROR1), namely ENST00000545203 or variant 3 (ROR1V3), was a predominant ROR1 transcript of neoplastic or normal cells in the Bioinformatic database, including GTEx and the 33 datasets from TCGA. Unlike the full-length ROR1 transcript, Drs. John and Ford deduced that ROR1V3 encoded a cytoplasmic ROR1 protein lacking an apparent signal peptide necessary for transport to the cell surface, which they presumed made it unlikely to function as a surface receptor for Wingless/Integrated (Wnt) factors. Moreover, they speculated that studies evaluating ROR1 via immunohistochemistry using any one of several anti-ROR1 mA2024-01-01T00:00:00Z2024 Jul2026-04-08T18:48:45.051Z2025-05-29T22:29:20.34ZS-EPMC112743623906214610.3390/biomedicines12071573