biostudies-literatureLi YNatural Science Foundation of Guangdong ProvinceScience and Technology Planning Project of Shenzhen Municipality873https://www.ebi.ac.uk/biostudies/studies/S-EPMC11274912biostudies-literatureUnknown14(7)Guanylate binding protein 5 (GBP5) is an emerging immune component that has been increasingly recognized for its involvement in autoimmune diseases, particularly inflammatory bowel disease (IBD). IBD is a complex disease involving inflammation of the gastrointestinal tract. Here, we explored the functional significance of GBP5 using <i>Gbp5</i> knockout mice and wildtype mice exposed to dextran sulfate sodium (DSS) to generate chronic colitis model. We found that <i>Gbp5</i> deficiency protected mice from DSS-induced chronic colitis. Transcriptome analysis of colon tissues showed reduced immune responses in <i>Gbp5</i> knockout mice compared to those in corresponding wildtype mice. We further observed that after repeated DSS exposure, the gut microbiota was altered, both in wildtype mice aBiomoleculesDiminished Immune Response and Elevated Abundance in Gut Microbe Dubosiella in Mouse Models of Chronic Colitis with GBP5 Deficiency.PMC112749122021A1515012478JCYJ20190807160411245Hou LLiu YLi YLi SWang WWang JfalseDiminished Immune Response and Elevated Abundance in Gut Microbe Dubosiella in Mouse Models of Chronic Colitis with GBP5 Deficiency.Guanylate binding protein 5 (GBP5) is an emerging immune component that has been increasingly recognized for its involvement in autoimmune diseases, particularly inflammatory bowel disease (IBD). IBD is a complex disease involving inflammation of the gastrointestinal tract. Here, we explored the functional significance of GBP5 using <i>Gbp5</i> knockout mice and wildtype mice exposed to dextran sulfate sodium (DSS) to generate chronic colitis model. We found that <i>Gbp5</i> deficiency protected mice from DSS-induced chronic colitis. Transcriptome analysis of colon tissues showed reduced immune responses in <i>Gbp5</i> knockout mice compared to those in corresponding wildtype mice. We further observed that after repeated DSS exposure, the gut microbiota was altered, both in wildtype mice a2024-01-01T00:00:00Z2024 Jul2025-08-27T03:11:00.875Z2025-08-27T03:11:00.875ZS-EPMC112749123906258810.3390/biom14070873