{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Chen D"],"funding":["NIDA NIH HHS","NIH HHS"],"pubmed_abstract":["Affordable sequencing and genotyping methods are essential for large scale genome-wide association studies. While genotyping microarrays and reference panels for imputation are available for human subjects, non-human model systems often lack such options. Our lab previously demonstrated an efficient and cost-effective method to genotype heterogeneous stock rats using double-digest genotyping-by-sequencing. However, low-coverage whole-genome sequencing offers an alternative method that has several advantages. Here, we describe a cost-effective, high-throughput, high-accuracy genotyping method for N/NIH heterogeneous stock rats that can use a combination of sequencing data previously generated by double-digest genotyping-by-sequencing and more recently generated by low-coverage whole-genome-"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.07.17.603984"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11275765"],"repository":["biostudies-literature"],"pubmed_title":["A Cost-effective, High-throughput, Highly Accurate Genotyping Method for Outbred Populations."],"pmcid":["PMC11275765"],"funding_grant_id":["P50 DA037844","S10 OD026929"],"pubmed_authors":["Okamoto F","Johnson BB","Cohen K","Sanches TM","Polesskaya O","Ziegler KS","Chen D","Lin B","Peng B","Chitre AS","Cheng R","Palmer AA","Nguyen KH"],"additional_accession":[]},"is_claimable":false,"name":"A Cost-effective, High-throughput, Highly Accurate Genotyping Method for Outbred Populations.","description":"Affordable sequencing and genotyping methods are essential for large scale genome-wide association studies. While genotyping microarrays and reference panels for imputation are available for human subjects, non-human model systems often lack such options. Our lab previously demonstrated an efficient and cost-effective method to genotype heterogeneous stock rats using double-digest genotyping-by-sequencing. However, low-coverage whole-genome sequencing offers an alternative method that has several advantages. Here, we describe a cost-effective, high-throughput, high-accuracy genotyping method for N/NIH heterogeneous stock rats that can use a combination of sequencing data previously generated by double-digest genotyping-by-sequencing and more recently generated by low-coverage whole-genome-","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2026-04-07T15:02:04.741Z","creation":"2025-04-03T23:55:49.499Z"},"accession":"S-EPMC11275765","cross_references":{"pubmed":["39071405"],"doi":["10.1101/2024.07.17.603984"]}}