{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Chang YT"],"funding":["NINDS NIH HHS","NIGMS NIH HHS","NIH HHS"],"pubmed_abstract":["Most of the mitochondria proteome is nuclear-encoded, synthesized by cytoplasmic ribosomes, and targeted to mitochondria post-translationally. However, a subset of mitochondrial-targeted proteins is imported co-translationally, although the molecular mechanisms governing this process remain unclear. We employ cellular cryo-electron tomography to visualize interactions between cytoplasmic ribosomes and mitochondria in <i>Saccharomyces cerevisiae</i>. We use surface morphometrics tools to identify a subset of ribosomes optimally oriented on mitochondrial membranes for protein import. This allows us to establish the first subtomogram average structure of a cytoplasmic ribosome on the surface of the mitochondria in the native cellular context, which showed three distinct connections with the o"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.07.17.604013"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11275913"],"repository":["biostudies-literature"],"pubmed_title":["Cytoplasmic ribosomes on mitochondria alter the local membrane environment for protein import."],"pmcid":["PMC11275913"],"funding_grant_id":["RF1 NS125674","R35 GM128798","S10 OD032467"],"pubmed_authors":["Rahmani H","Chang YT","Grotjahn DA","Barad BA","Zid BM"],"additional_accession":[]},"is_claimable":false,"name":"Cytoplasmic ribosomes on mitochondria alter the local membrane environment for protein import.","description":"Most of the mitochondria proteome is nuclear-encoded, synthesized by cytoplasmic ribosomes, and targeted to mitochondria post-translationally. However, a subset of mitochondrial-targeted proteins is imported co-translationally, although the molecular mechanisms governing this process remain unclear. We employ cellular cryo-electron tomography to visualize interactions between cytoplasmic ribosomes and mitochondria in <i>Saccharomyces cerevisiae</i>. We use surface morphometrics tools to identify a subset of ribosomes optimally oriented on mitochondrial membranes for protein import. This allows us to establish the first subtomogram average structure of a cytoplasmic ribosome on the surface of the mitochondria in the native cellular context, which showed three distinct connections with the o","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2026-04-07T21:19:30.922Z","creation":"2025-04-04T02:47:49.397Z"},"accession":"S-EPMC11275913","cross_references":{"pubmed":["39071314"],"doi":["10.1101/2024.07.17.604013"]}}