{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(7)"],"submitter":["Laranjeira ABA"],"funding":["Division of Cancer Treatment and Diagnosis, National Cancer Institute, Bethesda, MD, USA"],"pubmed_abstract":["<h4>Background</h4>Ten-eleven-translocation (TET) 2 is a member of the TET family of proteins (TET1-3). <i>DNMT1</i> gene deletion confers resistance to DNA methyltransferase (DNMT) inhibitors in colorectal, breast, and ovarian cancer cells. Currently, the effect of <i>DNMT1</i> gene status on TET2 phenotype following DNMT inhibitor treatment is unclear in human malignancies.<h4>Methods</h4>Human colorectal carcinoma HCT116 cells (<i>DNMT<sup>+/+</sup></i>) and their isogenic DNMT1 knockout (<i>DNMT1<sup>-/-</sup></i>) counterpart were treated with DNMT inhibitors. Expression of TET2 and tumor suppressor (p16<sup>ink4A</sup> and p15<sup>ink4B</sup>) proteins were examined by Western blot. Apoptosis and <i>CDKN2A</i> promoter demethylation following drug treatment were detected by Annexin-V"],"journal":["Diseases (Basel, Switzerland)"],"pagination":["163"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11276550"],"repository":["biostudies-literature"],"pubmed_title":["Upregulation of TET2 and Resistance to DNA Methyltransferase (DNMT) Inhibitors in <i>DNMT1</i>-Deleted Cancer Cells."],"pmcid":["PMC11276550"],"pubmed_authors":["Doroshow JH","Nguyen D","Laranjeira ABA","Yang SX","Pelosof LC"],"additional_accession":[]},"is_claimable":false,"name":"Upregulation of TET2 and Resistance to DNA Methyltransferase (DNMT) Inhibitors in <i>DNMT1</i>-Deleted Cancer Cells.","description":"<h4>Background</h4>Ten-eleven-translocation (TET) 2 is a member of the TET family of proteins (TET1-3). <i>DNMT1</i> gene deletion confers resistance to DNA methyltransferase (DNMT) inhibitors in colorectal, breast, and ovarian cancer cells. Currently, the effect of <i>DNMT1</i> gene status on TET2 phenotype following DNMT inhibitor treatment is unclear in human malignancies.<h4>Methods</h4>Human colorectal carcinoma HCT116 cells (<i>DNMT<sup>+/+</sup></i>) and their isogenic DNMT1 knockout (<i>DNMT1<sup>-/-</sup></i>) counterpart were treated with DNMT inhibitors. Expression of TET2 and tumor suppressor (p16<sup>ink4A</sup> and p15<sup>ink4B</sup>) proteins were examined by Western blot. Apoptosis and <i>CDKN2A</i> promoter demethylation following drug treatment were detected by Annexin-V","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2026-04-08T19:18:57.588Z","creation":"2026-04-08T12:59:24.196Z"},"accession":"S-EPMC11276550","cross_references":{"pubmed":["39057134"],"doi":["10.3390/diseases12070163"]}}