biostudies-literatureUnknown12(7)Laranjeira ABADivision of Cancer Treatment and Diagnosis, National Cancer Institute, Bethesda, MD, USA<h4>Background</h4>Ten-eleven-translocation (TET) 2 is a member of the TET family of proteins (TET1-3). <i>DNMT1</i> gene deletion confers resistance to DNA methyltransferase (DNMT) inhibitors in colorectal, breast, and ovarian cancer cells. Currently, the effect of <i>DNMT1</i> gene status on TET2 phenotype following DNMT inhibitor treatment is unclear in human malignancies.<h4>Methods</h4>Human colorectal carcinoma HCT116 cells (<i>DNMT^+/+</i>) and their isogenic DNMT1 knockout (<i>DNMT1^-/-</i>) counterpart were treated with DNMT inhibitors. Expression of TET2 and tumor suppressor (p16^ink4A and p15^ink4B) proteins were examined by Western blot. Apoptosis and <i>CDKN2A</i> promoter demethylation following drug treatment were detected by Annexin-VDiseases (Basel, Switzerland)163https://www.ebi.ac.uk/biostudies/studies/S-EPMC11276550biostudies-literatureUpregulation of TET2 and Resistance to DNA Methyltransferase (DNMT) Inhibitors in <i>DNMT1</i>-Deleted Cancer Cells.PMC11276550Doroshow JHNguyen DLaranjeira ABAYang SXPelosof LCfalseUpregulation of TET2 and Resistance to DNA Methyltransferase (DNMT) Inhibitors in <i>DNMT1</i>-Deleted Cancer Cells.<h4>Background</h4>Ten-eleven-translocation (TET) 2 is a member of the TET family of proteins (TET1-3). <i>DNMT1</i> gene deletion confers resistance to DNA methyltransferase (DNMT) inhibitors in colorectal, breast, and ovarian cancer cells. Currently, the effect of <i>DNMT1</i> gene status on TET2 phenotype following DNMT inhibitor treatment is unclear in human malignancies.<h4>Methods</h4>Human colorectal carcinoma HCT116 cells (<i>DNMT^+/+</i>) and their isogenic DNMT1 knockout (<i>DNMT1^-/-</i>) counterpart were treated with DNMT inhibitors. Expression of TET2 and tumor suppressor (p16^ink4A and p15^ink4B) proteins were examined by Western blot. Apoptosis and <i>CDKN2A</i> promoter demethylation following drug treatment were detected by Annexin-V2024-01-01T00:00:00Z2024 Jul2026-04-08T19:18:57.588Z2026-04-08T12:59:24.196ZS-EPMC112765503905713410.3390/diseases12070163