biostudies-literatureUnknown25(14)Yuan BDuring estrus, the poll glands of male Bactrian Camels (<i>Camelus Bactrianus</i>) become slightly raised, exuding a large amount of pale yellow watery secretion with a characteristic odor that may contain hydrogen sulfide (H₂S). However, whether H₂S can be synthesized in the poll glands of male Bactrian Camels and its role in inducing camel estrus remains unclear. This study aimed to identify differentially expressed proteins (DEPs) and signaling pathways in the poll gland tissues of male Bactrian Camels using data independent acquisition (DIA) proteomics. Additionally, gas chromatography-mass spectrometry (GC-MS) was performed to identify differentially expressed metabolites (DEMs) in the neck hair containing secretions during estrus in male Bactrian Camels, to explInternational journal of molecular sciences7700https://www.ebi.ac.uk/biostudies/studies/S-EPMC11276878biostudies-literatureExploring the Mechanism of H₂S Synthesis in Male Bactrian Camel Poll Glands Based on Data Independent Acquisition Proteomics and Non-Targeted Metabolomics.PMC11276878Dong WLi JYuan BZhang YChen WGao YZhang BZhang QZhao YZhao XfalseExploring the Mechanism of H₂S Synthesis in Male Bactrian Camel Poll Glands Based on Data Independent Acquisition Proteomics and Non-Targeted Metabolomics.During estrus, the poll glands of male Bactrian Camels (<i>Camelus Bactrianus</i>) become slightly raised, exuding a large amount of pale yellow watery secretion with a characteristic odor that may contain hydrogen sulfide (H₂S). However, whether H₂S can be synthesized in the poll glands of male Bactrian Camels and its role in inducing camel estrus remains unclear. This study aimed to identify differentially expressed proteins (DEPs) and signaling pathways in the poll gland tissues of male Bactrian Camels using data independent acquisition (DIA) proteomics. Additionally, gas chromatography-mass spectrometry (GC-MS) was performed to identify differentially expressed metabolites (DEMs) in the neck hair containing secretions during estrus in male Bactrian Camels, to expl2024-01-01T00:00:00Z2024 Jul2026-05-28T03:10:06.437Z2026-05-28T03:06:29.737ZS-EPMC112768783906294210.3390/ijms25147700