biostudies-literatureUnknown12(7)Zeng XSubgroup J avian leukosis virus (<b>ALV-J</b>) is a major pathogen in poultry, causing substantial economic losses to the poultry industry worldwide. Exosomal small RNAs derived from virus-infected cells or biological fluids can serve as viral transmission vectors. However, the role and mechanism of exosomal miRNA in ALV-J infection are unclear. In this study, we demonstrated that exosomal microRNA-7-25207 (<b>miR-7-25207</b>) could increase the titers of ALV-J. Exosomes isolated from ALV-J-infected DF-1 cells (<b>Exo-ALV-J</b>) contained partial viral proteins from ALV-J and could transmit the infection to uninfected DF-1 cells, leading to productive infection. Additionally, the RNA expression profile of exosomes was altered following ALV-J infection. miRNA analysis revealed that the exprMicroorganisms1495https://www.ebi.ac.uk/biostudies/studies/S-EPMC11279298biostudies-literatureExosomal miR-7-25207 Increases Subgroup J Avian Leukosis Virus Titers by Targeting the Akt-CyclinQ1 and PRC1-YAF2 Dual Pathways.PMC11279298Dong XChen LZeng XLiu TKong JXie QDai ZLi YLiao LFeng KTang SWong YHChen SfalseExosomal miR-7-25207 Increases Subgroup J Avian Leukosis Virus Titers by Targeting the Akt-CyclinQ1 and PRC1-YAF2 Dual Pathways.Subgroup J avian leukosis virus (<b>ALV-J</b>) is a major pathogen in poultry, causing substantial economic losses to the poultry industry worldwide. Exosomal small RNAs derived from virus-infected cells or biological fluids can serve as viral transmission vectors. However, the role and mechanism of exosomal miRNA in ALV-J infection are unclear. In this study, we demonstrated that exosomal microRNA-7-25207 (<b>miR-7-25207</b>) could increase the titers of ALV-J. Exosomes isolated from ALV-J-infected DF-1 cells (<b>Exo-ALV-J</b>) contained partial viral proteins from ALV-J and could transmit the infection to uninfected DF-1 cells, leading to productive infection. Additionally, the RNA expression profile of exosomes was altered following ALV-J infection. miRNA analysis revealed that the expr2024-01-01T00:00:00Z2024 Jul2026-06-30T03:10:48.147Z2025-04-19T13:12:56.345ZS-EPMC112792983906526310.3390/microorganisms12071495