biostudies-literatureWang JResearch Project of Jinan Microecological Biomedicine Shandong LaboratoryShandong Provincial Laboratory Projectthe Fundamental Research Funds for the Central Universities2343https://www.ebi.ac.uk/biostudies/studies/S-EPMC11279615biostudies-literatureUnknown16(14)Hesperetin (HT) is a type of citrus flavonoid with various pharmacological activities, including anti-tumor, anti-inflammation, antioxidant, and neuroprotective properties. However, the role and mechanism of HT in ulcerative colitis (UC) have been rarely studied. Our study aimed to uncover the beneficial effects of HT and its detailed mechanism in UC. Experimental colitis was induced by 2.5% dextran sodium sulfate (DSS) for seven days. HT ameliorated DSS-induced colitis in mice, showing marked improvement in weight loss, colon length, colonic pathological severity, and the levels of TNFα and IL6 in serum. A combination of informatics, network pharmacology, and molecular docking identified eight key targets and multi-pathways influenced by HT in UC. As a highlight, the experimental validatiNutrientsHesperetin Alleviated Experimental Colitis via Regulating Ferroptosis and Gut Microbiota.PMC11279615SYS2022022022ZFJH003JNL-2022001AYao YShi QYao TZeng YWang JLi LfalseHesperetin Alleviated Experimental Colitis via Regulating Ferroptosis and Gut Microbiota.Hesperetin (HT) is a type of citrus flavonoid with various pharmacological activities, including anti-tumor, anti-inflammation, antioxidant, and neuroprotective properties. However, the role and mechanism of HT in ulcerative colitis (UC) have been rarely studied. Our study aimed to uncover the beneficial effects of HT and its detailed mechanism in UC. Experimental colitis was induced by 2.5% dextran sodium sulfate (DSS) for seven days. HT ameliorated DSS-induced colitis in mice, showing marked improvement in weight loss, colon length, colonic pathological severity, and the levels of TNFα and IL6 in serum. A combination of informatics, network pharmacology, and molecular docking identified eight key targets and multi-pathways influenced by HT in UC. As a highlight, the experimental validati2024-01-01T00:00:00Z2024 Jul2026-04-08T18:55:34.359Z2025-04-19T13:11:32.309ZS-EPMC112796153906478610.3390/nu16142343