{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yu R"],"funding":["Fudan University","National Natural Science Foundation of China"],"pagination":["422-433"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11279724"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["39(3)"],"pubmed_abstract":["The utilization of enteroviruses engineered with reporter genes serves as a valuable tool for advancing our understanding of enterovirus biology and its applications, enabling the development of effective therapeutic and preventive strategies. In this study, our initial attempts to introduce a NanoLuc luciferase (NLuc) reporter gene into recombinant enteroviruses were unsuccessful in rescuing viable progenies. We hypothesized that the size of the inserted tag might be a determining factor in the rescue of the virus. Therefore, we inserted the 11-amino-acid HiBiT tag into the genomes of enterovirus A71 (EV-A71), coxsackievirus A10 (CVA10), coxsackievirus A7 (CVA7), coxsackievirus A16 (CVA16), namely EV-A71-HiBiT, CVA16-HiBiT, CVA10-HiBiT, CVA7-HiBiT, and observed that the HiBiT-tagged virus"],"journal":["Virologica Sinica"],"pubmed_title":["Integration of HiBiT into enteroviruses: A universal tool for advancing enterovirus virology research."],"pmcid":["PMC11279724"],"funding_grant_id":["82002135","82172250"],"pubmed_authors":["Li X","Xu M","Yan J","Yu R","Chenli Qiu","Zhang X","Shu J","Miaomiao Kang","Zhang S","Zhao J","Xu J","Zhang P"],"additional_accession":[]},"is_claimable":false,"name":"Integration of HiBiT into enteroviruses: A universal tool for advancing enterovirus virology research.","description":"The utilization of enteroviruses engineered with reporter genes serves as a valuable tool for advancing our understanding of enterovirus biology and its applications, enabling the development of effective therapeutic and preventive strategies. In this study, our initial attempts to introduce a NanoLuc luciferase (NLuc) reporter gene into recombinant enteroviruses were unsuccessful in rescuing viable progenies. We hypothesized that the size of the inserted tag might be a determining factor in the rescue of the virus. Therefore, we inserted the 11-amino-acid HiBiT tag into the genomes of enterovirus A71 (EV-A71), coxsackievirus A10 (CVA10), coxsackievirus A7 (CVA7), coxsackievirus A16 (CVA16), namely EV-A71-HiBiT, CVA16-HiBiT, CVA10-HiBiT, CVA7-HiBiT, and observed that the HiBiT-tagged virus","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jun","modification":"2026-06-02T23:12:18.279Z","creation":"2025-04-19T13:12:12.028Z"},"accession":"S-EPMC11279724","cross_references":{"pubmed":["38499155"],"doi":["10.1016/j.virs.2024.03.004"]}}