biostudies-literatureYu RFudan UniversityNational Natural Science Foundation of China422-433https://www.ebi.ac.uk/biostudies/studies/S-EPMC11279724biostudies-literatureUnknown39(3)The utilization of enteroviruses engineered with reporter genes serves as a valuable tool for advancing our understanding of enterovirus biology and its applications, enabling the development of effective therapeutic and preventive strategies. In this study, our initial attempts to introduce a NanoLuc luciferase (NLuc) reporter gene into recombinant enteroviruses were unsuccessful in rescuing viable progenies. We hypothesized that the size of the inserted tag might be a determining factor in the rescue of the virus. Therefore, we inserted the 11-amino-acid HiBiT tag into the genomes of enterovirus A71 (EV-A71), coxsackievirus A10 (CVA10), coxsackievirus A7 (CVA7), coxsackievirus A16 (CVA16), namely EV-A71-HiBiT, CVA16-HiBiT, CVA10-HiBiT, CVA7-HiBiT, and observed that the HiBiT-tagged virusVirologica SinicaIntegration of HiBiT into enteroviruses: A universal tool for advancing enterovirus virology research.PMC112797248200213582172250Li XXu MYan JYu RChenli QiuZhang XShu JMiaomiao KangZhang SZhao JXu JZhang PfalseIntegration of HiBiT into enteroviruses: A universal tool for advancing enterovirus virology research.The utilization of enteroviruses engineered with reporter genes serves as a valuable tool for advancing our understanding of enterovirus biology and its applications, enabling the development of effective therapeutic and preventive strategies. In this study, our initial attempts to introduce a NanoLuc luciferase (NLuc) reporter gene into recombinant enteroviruses were unsuccessful in rescuing viable progenies. We hypothesized that the size of the inserted tag might be a determining factor in the rescue of the virus. Therefore, we inserted the 11-amino-acid HiBiT tag into the genomes of enterovirus A71 (EV-A71), coxsackievirus A10 (CVA10), coxsackievirus A7 (CVA7), coxsackievirus A16 (CVA16), namely EV-A71-HiBiT, CVA16-HiBiT, CVA10-HiBiT, CVA7-HiBiT, and observed that the HiBiT-tagged virus2024-01-01T00:00:00Z2024 Jun2026-06-02T23:12:18.279Z2025-04-19T13:12:12.028ZS-EPMC112797243849915510.1016/j.virs.2024.03.004