biostudies-literatureUnknown15Guisasola-Serrano AAluminum hydroxide has long been employed as a vaccine adjuvant for its safety profile, although its precise mechanism of action remains elusive. In this study, we investigated the transcriptomic responses in sheep spleen following repetitive vaccination with aluminum adjuvanted vaccines and aluminum hydroxide alone. Notably, this work represents the first exploration of the sheep spleen transcriptome in such conditions. Animals were splitted in 3 treatment groups: vaccine group, adjuvant alone group and control group. A total of 18 high-depth RNA-seq libraries were sequenced, resulting in a rich dataset which also allowed isoform-level analysis. The comparisons between vaccine-treated and control groups (V vs C) as well as between vaccine-treated and adjuvant-alone groups (V vs A) revealeFrontiers in immunology1386590https://www.ebi.ac.uk/biostudies/studies/S-EPMC11284609biostudies-literatureIdentifying transcriptomic profiles in ovine spleen after repetitive vaccination.PMC11284609Varela-Martinez EBilbao-Arribas MGuisasola-Serrano AJugo BMAbendano NPerez MLujan LfalseIdentifying transcriptomic profiles in ovine spleen after repetitive vaccination.Aluminum hydroxide has long been employed as a vaccine adjuvant for its safety profile, although its precise mechanism of action remains elusive. In this study, we investigated the transcriptomic responses in sheep spleen following repetitive vaccination with aluminum adjuvanted vaccines and aluminum hydroxide alone. Notably, this work represents the first exploration of the sheep spleen transcriptome in such conditions. Animals were splitted in 3 treatment groups: vaccine group, adjuvant alone group and control group. A total of 18 high-depth RNA-seq libraries were sequenced, resulting in a rich dataset which also allowed isoform-level analysis. The comparisons between vaccine-treated and control groups (V vs C) as well as between vaccine-treated and adjuvant-alone groups (V vs A) reveale2024-01-01T00:00:00Z20242026-06-02T22:59:44.586Z2025-08-28T03:09:09.692ZS-EPMC112846093907698410.3389/fimmu.2024.1386590