biostudies-literatureDong NNational Major Science and Technology Projects of ChinaNational Natural Science Foundation of ChinaChinese Academy of Agricultural Sciences100893https://www.ebi.ac.uk/biostudies/studies/S-EPMC11287174biostudies-literatureUnknown5(7)Transitory starch is an important carbon source in leaves, and its biosynthesis and metabolism are closely related to grain quality and yield. The molecular mechanisms controlling leaf transitory starch biosynthesis and degradation and their effects on rice (Oryza sativa) quality and yield remain unclear. Here, we show that OsLESV and OsESV1, the rice orthologs of AtLESV and AtESV1, are associated with transitory starch biosynthesis in rice. The total starch and amylose contents in leaves and endosperms are significantly reduced, and the final grain quality and yield are compromised in oslesv and osesv1 single and oslesv esv1 double mutants. Furthermore, we found that OsLESV and OsESV1 bind to starch, and this binding depends on a highly conserved C-terminal tryptophan-rich region that actPlant communicationsOsLESV and OsESV1 promote transitory and storage starch biosynthesis to determine rice grain quality and yield.PMC1128717432188102CAAS-CACB-202402323720992023ZD04072Hu SShao GSheng ZMa LWei XDong NLu FWang HJiao GLi XLi SZhao SWang STang SCao RDuan YHu PfalseOsLESV and OsESV1 promote transitory and storage starch biosynthesis to determine rice grain quality and yield.Transitory starch is an important carbon source in leaves, and its biosynthesis and metabolism are closely related to grain quality and yield. The molecular mechanisms controlling leaf transitory starch biosynthesis and degradation and their effects on rice (Oryza sativa) quality and yield remain unclear. Here, we show that OsLESV and OsESV1, the rice orthologs of AtLESV and AtESV1, are associated with transitory starch biosynthesis in rice. The total starch and amylose contents in leaves and endosperms are significantly reduced, and the final grain quality and yield are compromised in oslesv and osesv1 single and oslesv esv1 double mutants. Furthermore, we found that OsLESV and OsESV1 bind to starch, and this binding depends on a highly conserved C-terminal tryptophan-rich region that act2024-01-01T00:00:00Z2024 Jul2025-04-22T14:53:15.628Z2025-04-06T01:07:48.346ZS-EPMC112871743858112810.1016/j.xplc.2024.100893