{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sumiyoshi R"],"funding":["MEXT | Japan Society for the Promotion of Science","MEXT | Japan Society for the Promotion of Science (JSPS)","Ministry of Education, Culture, Sports, Science and Technology","Ministry of Education, Culture, Sports, Science and Technology (MEXT)","Wellcome Trust"],"pagination":["e2403739121"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11287258"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["121(30)"],"pubmed_abstract":["Natural kinesin motors are tethered to their cargoes via short C-terminal or N-terminal linkers, whose docking against the core motor domain generates directional force. It remains unclear whether linker docking is the only process contributing directional force or whether linker docking is coupled to and amplifies an underlying, more fundamental force-generating mechanical cycle of the kinesin motor domain. Here, we show that kinesin motor domains tethered via double-stranded DNAs (dsDNAs) attached to surface loops drive robust microtubule (MT) gliding. Tethering using dsDNA attached to surface loops disconnects the C-terminal neck-linker and the N-terminal cover strand so that their dock-undock cycle cannot exert force. The most effective attachment positions for the dsDNA tether are loo"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Tether-scanning the kinesin motor domain reveals a core mechanical action."],"pmcid":["PMC11287258"],"funding_grant_id":["JP15K07022","JP23K18135","JP23KJ0813","23K14177","JP23H04401"],"pubmed_authors":["Furuta K","Sumiyoshi R","Yajima J","Nishizaka T","Cross RA","Furuta A","Yamagishi M"],"additional_accession":[]},"is_claimable":false,"name":"Tether-scanning the kinesin motor domain reveals a core mechanical action.","description":"Natural kinesin motors are tethered to their cargoes via short C-terminal or N-terminal linkers, whose docking against the core motor domain generates directional force. It remains unclear whether linker docking is the only process contributing directional force or whether linker docking is coupled to and amplifies an underlying, more fundamental force-generating mechanical cycle of the kinesin motor domain. Here, we show that kinesin motor domains tethered via double-stranded DNAs (dsDNAs) attached to surface loops drive robust microtubule (MT) gliding. Tethering using dsDNA attached to surface loops disconnects the C-terminal neck-linker and the N-terminal cover strand so that their dock-undock cycle cannot exert force. The most effective attachment positions for the dsDNA tether are loo","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2026-04-08T19:48:10.861Z","creation":"2025-04-06T01:15:30.233Z"},"accession":"S-EPMC11287258","cross_references":{"pubmed":["39012822"],"doi":["10.1073/pnas.2403739121"]}}