biostudies-literatureLukoszek RCancer Research UKEuropean Research CouncilMedical Research CouncilRoyal SocietyWellcome Trust114405https://www.ebi.ac.uk/biostudies/studies/S-EPMC11290353biostudies-literatureUnknown43(7)The RNA cap methyltransferase CMTR1 methylates the first transcribed nucleotide of RNA polymerase II transcripts, impacting gene expression mechanisms, including during innate immune responses. Using mass spectrometry, we identify a multiply phosphorylated region of CMTR1 (phospho-patch [P-Patch]), which is a substrate for the kinase CK2 (casein kinase II). CMTR1 phosphorylation alters intramolecular interactions, increases recruitment to RNA polymerase II, and promotes RNA cap methylation. P-Patch phosphorylation occurs during the G1 phase of the cell cycle, recruiting CMTR1 to RNA polymerase II during a period of rapid transcription and RNA cap formation. CMTR1 phosphorylation is required for the expression of specific RNAs, including ribosomal protein gene transcripts, and promotes cellCell reportsCK2 phosphorylation of CMTR1 promotes RNA cap formation and influenza virus infection.PMC11290353769080097462/Z/11/ZWRM\u2216R1\u2216180008219416/A/19/Z097945/Z/11/ZCTRQQR-2021\u2216100006A17196 / A31287MR/K024213/1Liang SBrett NJMLukoszek RPirillo CInesta-Vaquera FCowling VHRoberts EWHughes DJHepburn LAfalseCK2 phosphorylation of CMTR1 promotes RNA cap formation and influenza virus infection.The RNA cap methyltransferase CMTR1 methylates the first transcribed nucleotide of RNA polymerase II transcripts, impacting gene expression mechanisms, including during innate immune responses. Using mass spectrometry, we identify a multiply phosphorylated region of CMTR1 (phospho-patch [P-Patch]), which is a substrate for the kinase CK2 (casein kinase II). CMTR1 phosphorylation alters intramolecular interactions, increases recruitment to RNA polymerase II, and promotes RNA cap methylation. P-Patch phosphorylation occurs during the G1 phase of the cell cycle, recruiting CMTR1 to RNA polymerase II during a period of rapid transcription and RNA cap formation. CMTR1 phosphorylation is required for the expression of specific RNAs, including ribosomal protein gene transcripts, and promotes cell2024-01-01T00:00:00Z2024 Jul2026-06-01T16:20:02.903Z2025-04-19T07:05:25.057ZS-EPMC112903533892346310.1016/j.celrep.2024.114405