biostudies-literatureBing Cthe Fundamental Research Funds for the Central Universitiesthe Innovative Experiment Projects of Educational Ministry of China for Undergraduate771-782https://www.ebi.ac.uk/biostudies/studies/S-EPMC11290751biostudies-literatureUnknown19(9)<b>Aim:</b> The response of <i>E. coli</i> ATCC8739 to Brevinin-2CE (B2CE) was evaluated as a strategy to prevent the development of antimicrobial peptide (AMP)-resistant bacteria. <b>Methods:</b> Gene expression levels were detected by transcriptome sequencing and RT-PCR. Target genes were knocked out using CRISPR-Cas9. MIC was measured to evaluate strain resistance. <b>Results:</b> Expression of <i>acrZ</i> and <i>sugE</i> were increased with B2CE stimulation. ATCC8739Δ<i>acrZ</i> and ATCC8739Δ<i>sugE</i> showed twofold and fourfold increased sensitivity, respectively. The survival rate of ATCC8739 was reduced in the presence of B2CE/chlorpromazine (CPZ). Combinations of other AMPs with CPZ also showed antibacterial effects. <b>Conclusion:</b> The results indicate that combinations of AMPs/efflux pump inhibitors (EPIs) may be a potential approach to combat resistant bacteria.Future microbiologyEfflux pump inhibitor chlorpromazine effectively increases the susceptibility of <i>Escherichia coli</i> to antimicrobial peptide Brevinin-2CE.PMC11290751GK202302003S202310718118Mengjuan AZhi LChixin ZBing CYan SXinyu MXinyao TfalseEfflux pump inhibitor chlorpromazine effectively increases the susceptibility of <i>Escherichia coli</i> to antimicrobial peptide Brevinin-2CE.<b>Aim:</b> The response of <i>E. coli</i> ATCC8739 to Brevinin-2CE (B2CE) was evaluated as a strategy to prevent the development of antimicrobial peptide (AMP)-resistant bacteria. <b>Methods:</b> Gene expression levels were detected by transcriptome sequencing and RT-PCR. Target genes were knocked out using CRISPR-Cas9. MIC was measured to evaluate strain resistance. <b>Results:</b> Expression of <i>acrZ</i> and <i>sugE</i> were increased with B2CE stimulation. ATCC8739Δ<i>acrZ</i> and ATCC8739Δ<i>sugE</i> showed twofold and fourfold increased sensitivity, respectively. The survival rate of ATCC8739 was reduced in the presence of B2CE/chlorpromazine (CPZ). Combinations of other AMPs with CPZ also showed antibacterial effects. <b>Conclusion:</b> The results indicate that combinations of AMPs/efflux pump inhibitors (EPIs) may be a potential approach to combat resistant bacteria.2024-01-01T00:00:00Z2024 Jun2025-06-27T03:05:33.239Z2025-06-27T03:05:33.239ZS-EPMC112907513868316810.2217/fmb-2023-0272