biostudies-literatureChen Q2020 Natural Science Foundation of Fujian Province877-888https://www.ebi.ac.uk/biostudies/studies/S-EPMC11290754biostudies-literatureUnknown19(10)<b>Aim:</b> This study aims to explore the molecular mechanisms of cystic fibrosis (CF) complicated with nontuberculous mycobacteria (NTM) infection. <b>Materials & methods:</b> Expression profiles of CF with NTM-infected patients were downloaded from GEO database. Intersection analysis yielded 78 genes associated with CF with NTM infection. The protein-protein interaction (PPI) network and the functions of hub genes were investigated. <b>Results:</b> Five hub genes (<i>PIK3R1</i>, <i>IL1A</i>, <i>CXCR4</i>, <i>ACTN1</i>, <i>PFN1</i>) were identified, which were primarily enriched in actin-related biological processes and pathways. Transcription factors <i>RELA</i>, <i>JUN</i>, <i>NFKB1</i> and <i>FOS</i> that regulated hub genes modulated <i>IL1A</i> expression, while 21 other transcriptiFuture microbiologyMolecular mechanism analysis of nontuberculous mycobacteria infection in patients with cystic fibrosis.PMC112907542020J011146Chen QLi JfalseMolecular mechanism analysis of nontuberculous mycobacteria infection in patients with cystic fibrosis.<b>Aim:</b> This study aims to explore the molecular mechanisms of cystic fibrosis (CF) complicated with nontuberculous mycobacteria (NTM) infection. <b>Materials & methods:</b> Expression profiles of CF with NTM-infected patients were downloaded from GEO database. Intersection analysis yielded 78 genes associated with CF with NTM infection. The protein-protein interaction (PPI) network and the functions of hub genes were investigated. <b>Results:</b> Five hub genes (<i>PIK3R1</i>, <i>IL1A</i>, <i>CXCR4</i>, <i>ACTN1</i>, <i>PFN1</i>) were identified, which were primarily enriched in actin-related biological processes and pathways. Transcription factors <i>RELA</i>, <i>JUN</i>, <i>NFKB1</i> and <i>FOS</i> that regulated hub genes modulated <i>IL1A</i> expression, while 21 other transcripti2024-01-01T00:00:00Z2024 Jul2026-05-25T03:09:41.047Z2025-07-08T03:13:44.143ZS-EPMC112907543870028510.2217/fmb-2023-0237