biostudies-literatureObulkasim HNatural Science Foundation of Xinjiang Uygur Autonomous RegionXinjiang Uygur Autonomous Region Innovation Environment (Talents, Bases) Special Project4-17https://www.ebi.ac.uk/biostudies/studies/S-EPMC11290767biostudies-literatureUnknown18(1)Lim Domain and Actin Binding protein1 (lima1) influence cancer cell function. Thus far, functional role of lima1 in cholangiocarcinoma remains unknown. We used public databases, in vitro experiments, and multi-omics analysis to investigate the Lima1 in cholangiocarcinoma. Our results showed that lima1 expression is significantly upregulated and high levels of lima1 are significantly associated with vascular invasion in cholangiocarcinoma. Furthermore, lima1 knocking out inhibits the RBE cell invasion. Multi-omics data suggest that lima1 affect a broad spectrum of cancer related pathways, promoting tumor progression and metastatic ability in cholangiocarcinoma. This study provides insights into molecular associations of lima1 with tumorigenesist and establishes a preliminary picture of the Cell adhesion & migrationExpression and molecular insights of lima1 in cholangiocarcinoma.PMC112907672020Q0882021D01C240Liu YObulkasim HAdili ADuan SfalseExpression and molecular insights of lima1 in cholangiocarcinoma.Lim Domain and Actin Binding protein1 (lima1) influence cancer cell function. Thus far, functional role of lima1 in cholangiocarcinoma remains unknown. We used public databases, in vitro experiments, and multi-omics analysis to investigate the Lima1 in cholangiocarcinoma. Our results showed that lima1 expression is significantly upregulated and high levels of lima1 are significantly associated with vascular invasion in cholangiocarcinoma. Furthermore, lima1 knocking out inhibits the RBE cell invasion. Multi-omics data suggest that lima1 affect a broad spectrum of cancer related pathways, promoting tumor progression and metastatic ability in cholangiocarcinoma. This study provides insights into molecular associations of lima1 with tumorigenesist and establishes a preliminary picture of the 2024-01-01T00:00:00Z2024 Dec2026-07-15T08:07:34.51Z2025-05-29T19:06:51.463ZS-EPMC112907673907604310.1080/19336918.2024.2383068