{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Simwela NV"],"funding":["NIAID NIH HHS","NIH HHS"],"pubmed_abstract":["<i>Mycobacterium tuberculosis (Mtb)</i> infection of macrophages reprograms cellular metabolism to promote lipid retention. While it is clearly known that intracellular <i>Mtb</i> utilize host derived lipids to maintain infection, the role of macrophage lipid processing on the bacteria's ability to access the intracellular lipid pool remains undefined. We utilized a CRISPR-Cas9 genetic approach to assess the impact of sequential steps in fatty acid metabolism on the growth of intracellular <i>Mtb</i>. Our analyzes demonstrate that macrophages which cannot either import, store or catabolize fatty acids restrict <i>Mtb</i> growth by both common and divergent anti-microbial mechanisms, including increased glycolysis, increased oxidative stress, production of pro-inflammatory cytokines, enhanc"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.07.22.604660"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11291043"],"repository":["biostudies-literature"],"pubmed_title":["Impaired fatty acid import or catabolism in macrophages restricts intracellular growth of &lt;i&gt;Mycobacterium tuberculosis&lt;/i&gt;."],"pmcid":["PMC11291043"],"funding_grant_id":["S10 OD032135","R01 AI155319","U19 AI162598","T32 AI007349"],"pubmed_authors":["Simwela NV","Sassetti CM","Jaecklein E","Russell DG"],"additional_accession":[]},"is_claimable":false,"name":"Impaired fatty acid import or catabolism in macrophages restricts intracellular growth of &lt;i&gt;Mycobacterium tuberculosis&lt;/i&gt;.","description":"<i>Mycobacterium tuberculosis (Mtb)</i> infection of macrophages reprograms cellular metabolism to promote lipid retention. While it is clearly known that intracellular <i>Mtb</i> utilize host derived lipids to maintain infection, the role of macrophage lipid processing on the bacteria's ability to access the intracellular lipid pool remains undefined. We utilized a CRISPR-Cas9 genetic approach to assess the impact of sequential steps in fatty acid metabolism on the growth of intracellular <i>Mtb</i>. Our analyzes demonstrate that macrophages which cannot either import, store or catabolize fatty acids restrict <i>Mtb</i> growth by both common and divergent anti-microbial mechanisms, including increased glycolysis, increased oxidative stress, production of pro-inflammatory cytokines, enhanc","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Jan","modification":"2026-06-30T03:19:35.553Z","creation":"2025-04-06T01:57:07.037Z"},"accession":"S-EPMC11291043","cross_references":{"pubmed":["39091727"],"doi":["10.1101/2024.07.22.604660"]}}