biostudies-literatureUnknownFurlanis ENIA NIH HHSNIMH NIH HHSNINDS NIH HHSIn recent years, we and others have identified a number of enhancers that, when incorporated into rAAV vectors, can restrict the transgene expression to particular neuronal populations. Yet, viral tools to access and manipulate specific neuronal subtypes are still limited. Here, we performed systematic analysis of single cell genomic data to identify enhancer candidates for each of the telencephalic interneuron subtypes. We established a set of enhancer-AAV tools that are highly specific for distinct cortical interneuron populations and striatal cholinergic interneurons. These enhancers, when used in the context of different effectors, can target (fluorescent proteins), observe activity (GCaMP) and manipulate (opto-genetics) specific neuronal subtypes. We also validated our enhancer-AAV tools across species. Thus, we provide the field with a powerful set of tools to study neural circuits and functions and to develop precise and targeted therapy.bioRxiv : the preprint server for biology2024.07.17.603924https://www.ebi.ac.uk/biostudies/studies/S-EPMC11291062biostudies-literatureAn enhancer-AAV toolbox to target and manipulate distinct interneuron subtypes.PMC11291062U01 MH124602RF1 AG079269UH3 MH120096R21 NS133960U24 MH133236UF1 MH130701R01 AG085557Rice HHill SDu SNowakowski TJKaeser PSQazi AMacGregor CPLee ATTangen ACCaccavano ACummins ACLiebergall SDimidschstein JHanley EGoldberg EMBatista-Brito RYuan XWang YPlotnikova AVergara JRowan MJMAlvarez VAVallejo TRGorissen BLIbrahim LAHunt SXu QSara WillsNaskar STakesian AEMorabito AHyde LIqbal AFishell GFurlanis EOlah VJGarcia BLAzadi RAlyahyay MLee SRazzaq TAverbeck BBMcBain CJSjulson LFavuzzi EChittajallu RDwivedi DEldridge MAGShin JHChatain CPChang EFPelkey KAMcMahon JMcLean NBanks EVattino LGVlachos AHewitt LPereira AHairston AParanzino EKim SDai MHuang SVazquez ASoheib MVargish GMohanty ATran TAllaway KCBadiani RfalseAn enhancer-AAV toolbox to target and manipulate distinct interneuron subtypes.In recent years, we and others have identified a number of enhancers that, when incorporated into rAAV vectors, can restrict the transgene expression to particular neuronal populations. Yet, viral tools to access and manipulate specific neuronal subtypes are still limited. Here, we performed systematic analysis of single cell genomic data to identify enhancer candidates for each of the telencephalic interneuron subtypes. We established a set of enhancer-AAV tools that are highly specific for distinct cortical interneuron populations and striatal cholinergic interneurons. These enhancers, when used in the context of different effectors, can target (fluorescent proteins), observe activity (GCaMP) and manipulate (opto-genetics) specific neuronal subtypes. We also validated our enhancer-AAV tools across species. Thus, we provide the field with a powerful set of tools to study neural circuits and functions and to develop precise and targeted therapy.2025-01-01T00:00:00Z2025 Apr2026-04-08T15:35:00.92Z2025-04-04T01:21:05.486ZS-EPMC112910623909183510.1101/2024.07.17.603924