{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Franchini L"],"funding":["NIDCD NIH HHS","NHLBI NIH HHS"],"pubmed_abstract":["G protein-coupled receptors (GPCRs) are key pharmacological targets, yet many remain underutilized due to unknown activation mechanisms and ligands. Orphan GPCRs, lacking identified natural ligands, are a high priority for research, as identifying their ligands will aid in understanding their functions and potential as drug targets. Most GPCRs, including orphans, couple to G<sub>i/o/z</sub> family members, however current assays to detect their activation are limited, hindering ligand identification efforts. We introduce G<sub>Z</sub>ESTY, a highly sensitive, cell-based assay developed in an easily deliverable format designed to study the pharmacology of G<sub>i/o/z</sub>-coupled GPCRs and assist in deorphanization. We optimized assay conditions and developed an all-in-one vector employing"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2024.07.26.605282"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11291178"],"repository":["biostudies-literature"],"pubmed_title":["Gz Enhanced Signal Transduction assaY (G<sub>Z</sub>ESTY) for GPCR deorphanization."],"pmcid":["PMC11291178"],"funding_grant_id":["R01 DC022104","R01 HL153988"],"pubmed_authors":["Porter JJ","Lueck JD","Orlandi C","Franchini L"],"additional_accession":[]},"is_claimable":false,"name":"Gz Enhanced Signal Transduction assaY (G<sub>Z</sub>ESTY) for GPCR deorphanization.","description":"G protein-coupled receptors (GPCRs) are key pharmacological targets, yet many remain underutilized due to unknown activation mechanisms and ligands. Orphan GPCRs, lacking identified natural ligands, are a high priority for research, as identifying their ligands will aid in understanding their functions and potential as drug targets. Most GPCRs, including orphans, couple to G<sub>i/o/z</sub> family members, however current assays to detect their activation are limited, hindering ligand identification efforts. We introduce G<sub>Z</sub>ESTY, a highly sensitive, cell-based assay developed in an easily deliverable format designed to study the pharmacology of G<sub>i/o/z</sub>-coupled GPCRs and assist in deorphanization. We optimized assay conditions and developed an all-in-one vector employing","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2026-06-24T03:22:00.86Z","creation":"2024-10-17T23:03:34.29Z"},"accession":"S-EPMC11291178","cross_references":{"pubmed":["39091869"],"doi":["10.1101/2024.07.26.605282"]}}