{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["34(6)"],"submitter":["Yan B"],"pubmed_abstract":["Multiomics require concerted recording of independent information, ideally from a single experiment. In this study, we introduce RIMS-seq2, a high-throughput technique to simultaneously sequence genomes and overlay methylation information while requiring only a small modification of the experimental protocol for high-throughput DNA sequencing to include a controlled deamination step. Importantly, the rate of deamination of 5-methylcytosine is negligible and thus does not interfere with standard DNA sequencing and data processing. Thus, RIMS-seq2 libraries from whole- or targeted-genome sequencing show the same germline variation calling accuracy and sensitivity compared with standard DNA-seq. Additionally, regional methylation levels provide an accurate map of the human methylome."],"journal":["Genome research"],"pagination":["904-913"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11293541"],"repository":["biostudies-literature"],"pubmed_title":["Simultaneous assessment of human genome and methylome data in a single experiment using limited deamination of methylated cytosine."],"pmcid":["PMC11293541"],"pubmed_authors":["Ettwiller L","Wang D","Yan B"],"additional_accession":[]},"is_claimable":false,"name":"Simultaneous assessment of human genome and methylome data in a single experiment using limited deamination of methylated cytosine.","description":"Multiomics require concerted recording of independent information, ideally from a single experiment. In this study, we introduce RIMS-seq2, a high-throughput technique to simultaneously sequence genomes and overlay methylation information while requiring only a small modification of the experimental protocol for high-throughput DNA sequencing to include a controlled deamination step. Importantly, the rate of deamination of 5-methylcytosine is negligible and thus does not interfere with standard DNA sequencing and data processing. Thus, RIMS-seq2 libraries from whole- or targeted-genome sequencing show the same germline variation calling accuracy and sensitivity compared with standard DNA-seq. Additionally, regional methylation levels provide an accurate map of the human methylome.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2026-05-02T12:31:55.527Z","creation":"2024-12-03T19:34:20.36Z"},"accession":"S-EPMC11293541","cross_references":{"pubmed":["38858087"],"doi":["10.1101/gr.278294.123"]}}