{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mei F"],"funding":["the Key Project of Hubei Province","National Natural Science Foundation of China","China Postdoctoral Science Foundation","the China Postdoctoral Science Foundation","the National Key R&D Program of China","National Key Research and Development Program of China","the National Natural Science Foundation of China"],"pagination":["e13640"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11294424"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["57(8)"],"pubmed_abstract":["Macrophages play a pivotal role in the immunological cascade activated in response to biomedical implants, which predetermine acceptance or rejection of implants by the host via pro- and anti-inflammatory polarisation states. The role of chemical signals in macrophage polarisation is well-established, but how physical cues regulate macrophage function that may play a fundamental role in implant-bone interface, remains poorly understood. Here we find that bone marrow-derived macrophages (BMDM) cultured on polyacrylamide gels of varying stiffness exhibit different polarisation states. BMDM are 'primed' to a pro-inflammatory M1 phenotype on stiff substrates, while to an anti-inflammatory M2 phenotype on soft and medium stiffness substrates. It is further observed that matrix stiffening increa"],"journal":["Cell proliferation"],"pubmed_title":["Matrix stiffness regulates macrophage polarisation via the Piezo1-YAP signalling axis."],"pmcid":["PMC11294424"],"funding_grant_id":["2021M700279","2023T160029","2021YFC2400400/04","2020BCA072","82201123","82221003"],"pubmed_authors":["Guo Y","Chen L","Xie M","Zhou Y","Zhang S","Yang C","Ding S","Huang X","Mei F","Wang Y","Heng BC","Liu F","Deng X"],"additional_accession":[]},"is_claimable":false,"name":"Matrix stiffness regulates macrophage polarisation via the Piezo1-YAP signalling axis.","description":"Macrophages play a pivotal role in the immunological cascade activated in response to biomedical implants, which predetermine acceptance or rejection of implants by the host via pro- and anti-inflammatory polarisation states. The role of chemical signals in macrophage polarisation is well-established, but how physical cues regulate macrophage function that may play a fundamental role in implant-bone interface, remains poorly understood. Here we find that bone marrow-derived macrophages (BMDM) cultured on polyacrylamide gels of varying stiffness exhibit different polarisation states. BMDM are 'primed' to a pro-inflammatory M1 phenotype on stiff substrates, while to an anti-inflammatory M2 phenotype on soft and medium stiffness substrates. It is further observed that matrix stiffening increa","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Aug","modification":"2026-06-03T00:37:57.372Z","creation":"2025-02-19T02:27:29.908Z"},"accession":"S-EPMC11294424","cross_references":{"pubmed":["38556840"],"doi":["10.1111/cpr.13640"]}}