biostudies-literatureUnknownLi MCAMS Innovation Fund for Medical SciencesNational Natural Science Foundation of ChinaNational Key Research and Development Program of ChinaAneuploidy frequently occurs in cancer and developmental diseases such as Down syndrome, with its functional consequences implicated in dosage effects on gene expression and global perturbation of stress response and cell proliferation pathways. However, how aneuploidy affects spatial genome organization remains less understood. In this study, we addressed this question by utilizing the previously established isogenic wild-type (WT) and trisomic mouse embryonic stem cells (mESCs). We employed a combination of Hi-C, RNA-seq, chromosome painting and nascent RNA imaging technologies to compare the spatial genome structures and gene transcription among these cells. We found that trisomy has little effect on spatial genome organization at the level of A/B compartment or topologically associatinCell proliferatione13639https://www.ebi.ac.uk/biostudies/studies/S-EPMC11294443biostudies-literatureThe effect of trisomic chromosomes on spatial genome organization and global transcription in embryonic stem cells.PMC112944432021-I2M-1-0192021YFA11003002016-I2M-3-00232288102320250062016YFA0100103Liu YYang JHuang YLi CHu JLi TWu PZhang MSun YLi MXiao RfalseThe effect of trisomic chromosomes on spatial genome organization and global transcription in embryonic stem cells.Aneuploidy frequently occurs in cancer and developmental diseases such as Down syndrome, with its functional consequences implicated in dosage effects on gene expression and global perturbation of stress response and cell proliferation pathways. However, how aneuploidy affects spatial genome organization remains less understood. In this study, we addressed this question by utilizing the previously established isogenic wild-type (WT) and trisomic mouse embryonic stem cells (mESCs). We employed a combination of Hi-C, RNA-seq, chromosome painting and nascent RNA imaging technologies to compare the spatial genome structures and gene transcription among these cells. We found that trisomy has little effect on spatial genome organization at the level of A/B compartment or topologically associatin2024-01-01T00:00:00Z2024 Mar2025-04-19T20:45:58.429Z2025-02-19T02:27:19.295ZS-EPMC112944433855379610.1111/cpr.13639