{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Carlier F"],"funding":["Agence Nationale de la Recherche (ANR)","Agence Nationale de la Recherche"],"pagination":["e2402944121"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11295056"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["121(31)"],"pubmed_abstract":["In eukaryotes, repetitive DNA can become silenced de novo, either transcriptionally or post-transcriptionally, by processes independent of strong sequence-specific cues. The mechanistic nature of such processes remains poorly understood. We found that in the fungus <i>Neurospora crassa</i>, de novo initiation of both transcriptional and post-transcriptional silencing was linked to perturbed chromatin, which was produced experimentally by the aberrant activity of transcription factors at the <i>tetO</i> operator array. Transcriptional silencing was mediated by canonical constitutive heterochromatin. On the other hand, post-transcriptional silencing resembled repeat-induced quelling but occurred normally when homologous recombination was inactivated. All silencing of the <i>tetO</i> array wa"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Remodeling of perturbed chromatin can initiate de novo transcriptional and post-transcriptional silencing."],"pmcid":["PMC11295056"],"funding_grant_id":["ANR-11-LABX-0044","ANR-19-CE12-0002","ANR-10-IDEX-0001-02","ANR-10-INBS-04","ANR-10-LABX-0062"],"pubmed_authors":["Carlier F","Castro Ramirez S","Kilani J","Taddei A","Chehboub S","Gladyshev E","Loiodice I"],"additional_accession":[]},"is_claimable":false,"name":"Remodeling of perturbed chromatin can initiate de novo transcriptional and post-transcriptional silencing.","description":"In eukaryotes, repetitive DNA can become silenced de novo, either transcriptionally or post-transcriptionally, by processes independent of strong sequence-specific cues. The mechanistic nature of such processes remains poorly understood. We found that in the fungus <i>Neurospora crassa</i>, de novo initiation of both transcriptional and post-transcriptional silencing was linked to perturbed chromatin, which was produced experimentally by the aberrant activity of transcription factors at the <i>tetO</i> operator array. Transcriptional silencing was mediated by canonical constitutive heterochromatin. On the other hand, post-transcriptional silencing resembled repeat-induced quelling but occurred normally when homologous recombination was inactivated. All silencing of the <i>tetO</i> array wa","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Jul","modification":"2026-06-02T06:49:26.22Z","creation":"2025-04-04T11:46:53.727Z"},"accession":"S-EPMC11295056","cross_references":{"pubmed":["39052837"],"doi":["10.1073/pnas.2402944121"]}}