{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Pakrashi S"],"funding":["University Grants Commission","ICMR","MAKAUT TEQIP"],"pagination":["tfae117"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11295208"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(4)"],"pubmed_abstract":["<h4>Aim</h4>This study is primarily designed to investigate the potential neuroprotective effects of polyphenol against 6-OHDAcaused neurotoxicity on SH-SY5Y cells.<h4>Materials and methods</h4>Cytotoxic effect of 6-OHDA and valuable role of quercetin, myricetin and kaempferol on SH-SY5Y cells were analyzed by MTT assay. Generation of 6-OHDA-stimulated reactive oxygen species (ROS) was measured using DCFDA fluorescence dye. Alteration of 6-OHDA-caused mitochondrial membrane potential and nuclear condensation was investigated with the help of rhodamine-123 and hoechest stain. Immunoblotting was performed to detect the expression level of 6-OHDA-caused alpha-synuclein (á-syn), Bcl-2 associated protein X (BAX), caspase 3, cleaved Poly ADP - ribose polymerase (PARP) and Bcell lymphoma 2 protei"],"journal":["Toxicology research"],"pubmed_title":["Quercetin alleviates 6-OHDA-caused apoptosis in SH-SY5Y cells."],"pmcid":["PMC11295208"],"funding_grant_id":["3/1/3/JRF-2013-HRD-10-40901","F/17-164/2013/SA-1"],"pubmed_authors":["Chakraborty J","Bandyopadhyay J","Pakrashi S"],"additional_accession":[]},"is_claimable":false,"name":"Quercetin alleviates 6-OHDA-caused apoptosis in SH-SY5Y cells.","description":"<h4>Aim</h4>This study is primarily designed to investigate the potential neuroprotective effects of polyphenol against 6-OHDAcaused neurotoxicity on SH-SY5Y cells.<h4>Materials and methods</h4>Cytotoxic effect of 6-OHDA and valuable role of quercetin, myricetin and kaempferol on SH-SY5Y cells were analyzed by MTT assay. Generation of 6-OHDA-stimulated reactive oxygen species (ROS) was measured using DCFDA fluorescence dye. Alteration of 6-OHDA-caused mitochondrial membrane potential and nuclear condensation was investigated with the help of rhodamine-123 and hoechest stain. Immunoblotting was performed to detect the expression level of 6-OHDA-caused alpha-synuclein (á-syn), Bcl-2 associated protein X (BAX), caspase 3, cleaved Poly ADP - ribose polymerase (PARP) and Bcell lymphoma 2 protei","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Aug","modification":"2025-08-12T03:05:15.058Z","creation":"2025-08-12T03:05:15.058Z"},"accession":"S-EPMC11295208","cross_references":{"pubmed":["39100858"],"doi":["10.1093/toxres/tfae117"]}}