biostudies-literaturePakrashi SUniversity Grants CommissionICMRMAKAUT TEQIPtfae117https://www.ebi.ac.uk/biostudies/studies/S-EPMC11295208biostudies-literatureUnknown13(4)<h4>Aim</h4>This study is primarily designed to investigate the potential neuroprotective effects of polyphenol against 6-OHDAcaused neurotoxicity on SH-SY5Y cells.<h4>Materials and methods</h4>Cytotoxic effect of 6-OHDA and valuable role of quercetin, myricetin and kaempferol on SH-SY5Y cells were analyzed by MTT assay. Generation of 6-OHDA-stimulated reactive oxygen species (ROS) was measured using DCFDA fluorescence dye. Alteration of 6-OHDA-caused mitochondrial membrane potential and nuclear condensation was investigated with the help of rhodamine-123 and hoechest stain. Immunoblotting was performed to detect the expression level of 6-OHDA-caused alpha-synuclein (á-syn), Bcl-2 associated protein X (BAX), caspase 3, cleaved Poly ADP - ribose polymerase (PARP) and Bcell lymphoma 2 proteiToxicology researchQuercetin alleviates 6-OHDA-caused apoptosis in SH-SY5Y cells.PMC112952083/1/3/JRF-2013-HRD-10-40901F/17-164/2013/SA-1Chakraborty JBandyopadhyay JPakrashi SfalseQuercetin alleviates 6-OHDA-caused apoptosis in SH-SY5Y cells.<h4>Aim</h4>This study is primarily designed to investigate the potential neuroprotective effects of polyphenol against 6-OHDAcaused neurotoxicity on SH-SY5Y cells.<h4>Materials and methods</h4>Cytotoxic effect of 6-OHDA and valuable role of quercetin, myricetin and kaempferol on SH-SY5Y cells were analyzed by MTT assay. Generation of 6-OHDA-stimulated reactive oxygen species (ROS) was measured using DCFDA fluorescence dye. Alteration of 6-OHDA-caused mitochondrial membrane potential and nuclear condensation was investigated with the help of rhodamine-123 and hoechest stain. Immunoblotting was performed to detect the expression level of 6-OHDA-caused alpha-synuclein (á-syn), Bcl-2 associated protein X (BAX), caspase 3, cleaved Poly ADP - ribose polymerase (PARP) and Bcell lymphoma 2 protei2024-01-01T00:00:00Z2024 Aug2025-08-12T03:05:15.058Z2025-08-12T03:05:15.058ZS-EPMC112952083910085810.1093/toxres/tfae117