{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lyu Y"],"funding":["Sichuan University (SCU)","National Natural Science Foundation of China","Sichuan University","National Natural Science Foundation of China (National Science Foundation of China)"],"pagination":["6511"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11297040"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(1)"],"pubmed_abstract":["Vesicular monoamine transporter 2 (VMAT2) belongs to the major facilitator superfamily (MFS), and mediates cytoplasmic monoamine packaging into presynaptic vesicles. Here, we present two cryo-EM structures of VMAT2, with a frog VMAT2 adopting a canonical MFS fold and an engineered sheep VMAT2 adopting a non-canonical fold. Both VMAT2 proteins mediate uptake of a selective fluorescent VMAT2 substrate into cells. Molecular docking, substrate binding and transport analysis reveal potential substrate binding mechanism in VMAT2. Meanwhile, caution is advised when interpreting engineered membrane protein structures."],"journal":["Nature communications"],"pubmed_title":["Engineering of a mammalian VMAT2 for cryo-EM analysis results in non-canonical protein folding."],"pmcid":["PMC11297040"],"funding_grant_id":["31770783","2023SCUH0067"],"pubmed_authors":["Fu C","Lyu Y","Ma H","Su Z","Zhou X","Sun Z"],"additional_accession":[]},"is_claimable":false,"name":"Engineering of a mammalian VMAT2 for cryo-EM analysis results in non-canonical protein folding.","description":"Vesicular monoamine transporter 2 (VMAT2) belongs to the major facilitator superfamily (MFS), and mediates cytoplasmic monoamine packaging into presynaptic vesicles. Here, we present two cryo-EM structures of VMAT2, with a frog VMAT2 adopting a canonical MFS fold and an engineered sheep VMAT2 adopting a non-canonical fold. Both VMAT2 proteins mediate uptake of a selective fluorescent VMAT2 substrate into cells. Molecular docking, substrate binding and transport analysis reveal potential substrate binding mechanism in VMAT2. Meanwhile, caution is advised when interpreting engineered membrane protein structures.","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Aug","modification":"2026-05-02T12:42:13.886Z","creation":"2025-02-19T02:27:30.91Z"},"accession":"S-EPMC11297040","cross_references":{"pubmed":["39095428"],"doi":["10.1038/s41467-024-50934-5"]}}