biostudies-literatureWy SMinistry of Science, ICT and Future Planning (MSIP)Rural Development Administration (RDA)Rural Development AdministrationMinistry of Science, ICT and Future Planning840https://www.ebi.ac.uk/biostudies/studies/S-EPMC11297928biostudies-literatureUnknown11(1)Recent advancements in sequencing and genome assembly technologies have led to rapid generation of high-quality genome assemblies for various species and breeds. Despite the importance as minipigs an animal model in biomedical research, the construction of high-quality genome assemblies of minipigs still lags behind other pig breeds. To address this problem, we constructed a high-quality chromosome-level genome assembly of the Korean minipig (KMP) utilizing multiple different types of sequencing reads and reference genomes. The KMP assembly included 19 chromosome-level sequences with a total length of 2.52 Gb and N50 of 137 Mb. Comparative analyses with the pig reference genome (Sscrofa11.1) demonstrated comparable contiguity and completeness of the KMP assembly. Additionally, genome annotScientific dataA chromosome-level genome assembly of the Korean minipig (Sus scrofa).PMC11297928NRF-2021M3H9A2097134NRF-2022R1F1A1065159PJ01334302Park WCho ICKwon DChai HHKim JWy SfalseA chromosome-level genome assembly of the Korean minipig (Sus scrofa).Recent advancements in sequencing and genome assembly technologies have led to rapid generation of high-quality genome assemblies for various species and breeds. Despite the importance as minipigs an animal model in biomedical research, the construction of high-quality genome assemblies of minipigs still lags behind other pig breeds. To address this problem, we constructed a high-quality chromosome-level genome assembly of the Korean minipig (KMP) utilizing multiple different types of sequencing reads and reference genomes. The KMP assembly included 19 chromosome-level sequences with a total length of 2.52 Gb and N50 of 137 Mb. Comparative analyses with the pig reference genome (Sscrofa11.1) demonstrated comparable contiguity and completeness of the KMP assembly. Additionally, genome annot2024-01-01T00:00:00Z2024 Aug2026-05-22T03:10:33.604Z2026-05-22T03:07:55.496ZS-EPMC112979283909764910.1038/s41597-024-03680-8