{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jacob AM"],"funding":["Universitätsklinikum Bonn","Deutsche Forschungsgemeinschaft"],"pagination":["18033"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11298517"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["Light sheet fluorescence microscopy (LSFM) is a transformative imaging method that enables the visualization of non-dissected specimen in real-time 3D. Optical clearing of tissues is essential for LSFM, typically employing toxic solvents. Here, we test the applicability of a non-hazardous alternative, ethyl cinnamate (ECi). We comprehensively characterized autofluorescence (AF) spectra in diverse murine tissues-ocular globe, knee, and liver-employing LSFM under various excitation wavelengths (405-785 nm) to test the feasibility of unstained samples for diagnostic purposes, in particular regarding percutaneous biopsies, as they constitute to most harvested type of tissue sample in clinical routine. Ocular globe structures were best discerned with 640 nm excitation. Knee tissue showed comple"],"journal":["Scientific reports"],"pubmed_title":["Autofluorescence-based tissue characterization enhances clinical prospects of light-sheet-microscopy."],"pmcid":["PMC11298517"],"funding_grant_id":["390873048"],"pubmed_authors":["Jacob AM","Abdullah Z","Bohner AMC","Salam B","Schildberg FA","Lindemann AF","Attenberger UI","Layer YC","Kuetting D","Wagenpfeil J","Panahabadi S","Wintergerst MWM","Geiger S","Kurt D"],"additional_accession":[]},"is_claimable":false,"name":"Autofluorescence-based tissue characterization enhances clinical prospects of light-sheet-microscopy.","description":"Light sheet fluorescence microscopy (LSFM) is a transformative imaging method that enables the visualization of non-dissected specimen in real-time 3D. Optical clearing of tissues is essential for LSFM, typically employing toxic solvents. Here, we test the applicability of a non-hazardous alternative, ethyl cinnamate (ECi). We comprehensively characterized autofluorescence (AF) spectra in diverse murine tissues-ocular globe, knee, and liver-employing LSFM under various excitation wavelengths (405-785 nm) to test the feasibility of unstained samples for diagnostic purposes, in particular regarding percutaneous biopsies, as they constitute to most harvested type of tissue sample in clinical routine. Ocular globe structures were best discerned with 640 nm excitation. Knee tissue showed comple","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Aug","modification":"2025-04-05T15:03:17.84Z","creation":"2025-02-19T04:40:35.572Z"},"accession":"S-EPMC11298517","cross_references":{"pubmed":["39098935"],"doi":["10.1038/s41598-024-67366-2"]}}