{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Rogers HT"],"funding":["NHLBI NIH HHS","Training Program in Translational Cardiovascular Science","National Institutes of Health","NIGMS NIH HHS","NIH HHS","National Science Foundation"],"pubmed_abstract":["Top-down-mass spectrometry (MS)-based proteomics has emerged as a premier technology to examine proteins at the proteoform level, enabling characterization of genetic mutations, alternative splicing, and post-translational modifications. However, significant challenges that remain in top-down proteomics include the analysis of large proteoforms and the sensitivity required to examine proteoforms from minimal amounts of sample. To address these challenges, we have developed a new method termed \"small-scale serial Size Exclusion Chromatography\" (s3SEC), which incorporates a small-scale protein extraction (1 mg of tissue) and serial SEC without postfractionation sample handling, coupled with online high sensitivity capillary reversed-phase "],"journal":["Analytical chemistry"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC11298573"],"repository":["biostudies-literature"],"pubmed_title":["Small-Scale Serial Size Exclusion Chromatography (s3SEC) for High Sensitivity Top-Down Proteomics of Large Proteoforms."],"pmcid":["PMC11298573"],"funding_grant_id":["R01 HL109810","T32 HL007936","R01 GM125085","DGE-1747503","S10 OD018475","R01 HL109810-06","T32 HL007936-21","R01 HL096971"],"pubmed_authors":["Rogers HT","Larson EJ","Fischer MS","Rossler KJ","Ge Y","Wang D","Gao Z","Alpert AJ","Melby JA","Ehlers LE"],"additional_accession":[]},"is_claimable":false,"name":"Small-Scale Serial Size Exclusion Chromatography (s3SEC) for High Sensitivity Top-Down Proteomics of Large Proteoforms.","description":"Top-down-mass spectrometry (MS)-based proteomics has emerged as a premier technology to examine proteins at the proteoform level, enabling characterization of genetic mutations, alternative splicing, and post-translational modifications. However, significant challenges that remain in top-down proteomics include the analysis of large proteoforms and the sensitivity required to examine proteoforms from minimal amounts of sample. To address these challenges, we have developed a new method termed \"small-scale serial Size Exclusion Chromatography\" (s3SEC), which incorporates a small-scale protein extraction (1 mg of tissue) and serial SEC without postfractionation sample handling, coupled with online high sensitivity capillary reversed-phase ","dates":{"release":"2024-01-01T00:00:00Z","publication":"2024 Feb","modification":"2026-03-15T22:44:36.64Z","creation":"2025-08-13T03:06:34.219Z"},"accession":"S-EPMC11298573","cross_references":{"pubmed":["38315630"],"doi":["10.1021/acs.analchem.3c05733"]}}