biostudies-literatureUnknown27Ren CUniaxial cyclic stretching plays a pivotal role in the fields of tissue engineering and regenerative medicine, influencing cell behaviors and functionality based on physical properties, including matrix morphology and mechanical stimuli. This study delves into the response of endothelial cells to uniaxial cyclic strain within the geometric constraints of micro-nano fibers. Various structural scaffold forms of poly(l-lactide-<i>co</i>-caprolactone) (PLCL), such as flat membranes, randomly oriented fiber membranes, and aligned fiber membranes, were fabricated through solvent casting and electrospinning methods. Our investigation focuses on the morphological variation of endothelial cells under diverse geometric constraints and the mechanical-dependent release of nitric oxide (NO) on orientedMaterials today. Bio101152https://www.ebi.ac.uk/biostudies/studies/S-EPMC11298614biostudies-literatureImpact of uniaxial cyclic stretching on matrix-associated endothelial cell responses.PMC11298614Li XWang DLi QWang XChang ZLi KRen CXu YfalseImpact of uniaxial cyclic stretching on matrix-associated endothelial cell responses.Uniaxial cyclic stretching plays a pivotal role in the fields of tissue engineering and regenerative medicine, influencing cell behaviors and functionality based on physical properties, including matrix morphology and mechanical stimuli. This study delves into the response of endothelial cells to uniaxial cyclic strain within the geometric constraints of micro-nano fibers. Various structural scaffold forms of poly(l-lactide-<i>co</i>-caprolactone) (PLCL), such as flat membranes, randomly oriented fiber membranes, and aligned fiber membranes, were fabricated through solvent casting and electrospinning methods. Our investigation focuses on the morphological variation of endothelial cells under diverse geometric constraints and the mechanical-dependent release of nitric oxide (NO) on oriented2024-01-01T00:00:00Z2024 Aug2025-04-05T11:10:09.019Z2025-04-05T11:10:09.019ZS-EPMC112986143910490110.1016/j.mtbio.2024.101152