biostudies-literatureUnknown45(4)Fu YTumor necrosis factor α (TNFα) exhibits diverse biological functions; however, its regulatory roles in myogenesis are not fully understood. In the present study, we explored the function of TNFα in myoblast proliferation, differentiation, migration, and myotube fusion in primary myoblasts and C2C12 cells. To this end, we constructed TNFα muscle-conditional knockout ( TNFα-CKO) mice and compared them with flox mice to assess the effects of TNFα knockout on skeletal muscles. Results indicated that TNFα-CKO mice displayed phenotypes such as accelerated muscle development, enhanced regenerative capacity, and improved exercise endurance compared to flox mice, with no significant differences observed in major visceral organs or skeletal structure.Zoological research951-960https://www.ebi.ac.uk/biostudies/studies/S-EPMC11298682biostudies-literatureTumor necrosis factor α deficiency promotes myogenesis and muscle regeneration.PMC11298682Shang PYan DWZhang HFu YHao XZhang BNie JRfalseTumor necrosis factor α deficiency promotes myogenesis and muscle regeneration.Tumor necrosis factor α (TNFα) exhibits diverse biological functions; however, its regulatory roles in myogenesis are not fully understood. In the present study, we explored the function of TNFα in myoblast proliferation, differentiation, migration, and myotube fusion in primary myoblasts and C2C12 cells. To this end, we constructed TNFα muscle-conditional knockout ( TNFα-CKO) mice and compared them with flox mice to assess the effects of TNFα knockout on skeletal muscles. Results indicated that TNFα-CKO mice displayed phenotypes such as accelerated muscle development, enhanced regenerative capacity, and improved exercise endurance compared to flox mice, with no significant differences observed in major visceral organs or skeletal structure.2024-01-01T00:00:00Z2024 Jul2026-06-01T15:25:13.33Z2025-02-19T04:40:27.522ZS-EPMC112986823902108310.24272/j.issn.2095-8137.2024.039